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Int. J. Mol. Sci. 2017, 18(10), 2189;

ZnT3 Gene Deletion Reduces Colchicine-Induced Dentate Granule Cell Degeneration

Department of Physiology, College of Medicine, Hallym University, Chuncheon 24252, Korea
Author to whom correspondence should be addressed.
Received: 29 September 2017 / Revised: 16 October 2017 / Accepted: 17 October 2017 / Published: 19 October 2017
(This article belongs to the Special Issue Zinc Signaling in Physiology and Pathogenesis)
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Our previous study demonstrated that colchicine-induced dentate granule cell death is caused by blocking axonal flow and the accumulation of intracellular zinc. Zinc is concentrated in the synaptic vesicles via zinc transporter 3 (ZnT3), which facilitates zinc transport from the cytosol into the synaptic vesicles. The aim of the present study was to identify the role of ZnT3 gene deletion on colchicine-induced dentate granule cell death. The present study used young (3–5 months) mice of the wild-type (WT) or the ZnT3/ genotype. Colchicine (10 µg/kg) was injected into the hippocampus, and then brain sections were evaluated 12 or 24 h later. Cell death was evaluated by Fluoro-Jade B; oxidative stress was analyzed by 4-hydroxy-2-nonenal; and dendritic damage was detected by microtubule-associated protein 2. Zinc accumulation was detected by N-(6-methoxy-8-quinolyl)-para-toluenesulfonamide (TSQ) staining. Here, we found that ZnT3/ reduced the number of degenerating cells after colchicine injection. The ZnT3/-mediated inhibition of cell death was accompanied by suppression of oxidative injury, dendritic damage and zinc accumulation. In addition, ZnT3/ mice showed more glutathione content than WT mice and inhibited neuronal glutathione depletion by colchicine. These findings suggest that increased neuronal glutathione by ZnT3 gene deletion prevents colchicine-induced dentate granule cell death. View Full-Text
Keywords: ZnT3; colchicine; axonal transport; zinc; neuron death; oxidative stress; glutathione ZnT3; colchicine; axonal transport; zinc; neuron death; oxidative stress; glutathione

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Choi, B.Y.; Hong, D.K.; Suh, S.W. ZnT3 Gene Deletion Reduces Colchicine-Induced Dentate Granule Cell Degeneration. Int. J. Mol. Sci. 2017, 18, 2189.

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