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Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

by Zhenjian Zhuo 1,†, Yuanyuan Yu 2,3,4,5,†, Maolin Wang 2,3,4,5, Jie Li 1, Zongkang Zhang 1, Jin Liu 2,3,4,5, Xiaohao Wu 2,3,4,5, Aiping Lu 2,3,4,5,*, Ge Zhang 2,3,4,5,* and Baoting Zhang 1,*
1
School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong 999077, China
2
Law Sau Fai Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
3
Institute of Integrated Bioinfomedicine and Translational Science, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
4
Institute of Precision Medicine and Innovative Drug Discovery, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
5
Shenzhen Lab of Combinatorial Compounds and Targeted Drug Delivery, HKBU Institute of Research and Continuing Education, Shenzhen 518000, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2017, 18(10), 2142; https://doi.org/10.3390/ijms18102142
Received: 20 September 2017 / Revised: 11 October 2017 / Accepted: 12 October 2017 / Published: 14 October 2017
(This article belongs to the Section Biochemistry)
Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents and therapeutic uses. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, few aptamer-based products have already successfully entered into clinical and industrial use. Besides, it is still a challenge to obtain aptamers with high affinity in a more efficient way. Thus, it is important to comprehensively review the current shortage and achievement of aptamer-related technology. In this review, we first present the limitations and notable advances of aptamer selection. Then, we compare the different methods used in the kinetic characterization of aptamers. We also discuss the impetus and developments of the clinical application of aptamers. View Full-Text
Keywords: aptamer; SELEX; selection; targeted drug delivery systems; therapy aptamer; SELEX; selection; targeted drug delivery systems; therapy
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MDPI and ACS Style

Zhuo, Z.; Yu, Y.; Wang, M.; Li, J.; Zhang, Z.; Liu, J.; Wu, X.; Lu, A.; Zhang, G.; Zhang, B. Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine. Int. J. Mol. Sci. 2017, 18, 2142.

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