Identification of Proteins Interacting with Cytoplasmic High-Mobility Group Box 1 during the Hepatocellular Response to Ischemia Reperfusion Injury
Abstract
:1. Introduction
2. Results
2.1. Enrichment of Cytoplasmic High-Mobility Group Box 1 (HMGB1)-Binding Protein Complex from Warm Ischemia Reperfusion (WI/R) Liver Tissue
2.2. Identification and Verification of Binding Proteins of Cytoplasmic HMGB1 in WI/R Liver Tissue
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Animals and Selective Liver Ischemia
4.3. Protein Extraction and Co-Immunoprecipitation
4.4. Two-Dimensional Electrophoresis and Silver Staining
4.5. Mass Spectrometry Detection
4.6. Western Blotting
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
IRI | Ischemia reperfusion injury |
WI/R | Warm ischemia reperfusion |
WB | Western blotting |
co-IP | Co-immunoprecipitation |
2DE/MS | 2-dimensional electrophoresis and mass spectrometry |
BHMT | Betaine–homocysteine S-methyltransferase 1 |
CTH | Cystathionine γ-lyase |
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Accession 1 | Protein | Region No. | Score | Coverage | No. of Peptides | MW(kDa) 2 | calc. pI 3 | Biological Function 4 |
---|---|---|---|---|---|---|---|---|
13540663 | Betaine—homocysteine S-methyltransferase 1 | 1 | 566.87 | 10.57 | 6 | 44.9 | 7.91 | Metabolic pathways; Cysteine and methionine metabolism |
13540663 | Betaine—homocysteine S-methyltransferase 1 | 2 | 566.74 | 16.46 | 10 | 44.9 | 7.91 | |
56030 | Cystathionine γ-lyase | 2 | 80.63 | 10.44 | 4 | 39.7 | 8.03 | Metabolic pathways; Cysteine and methionine metabolism; Sulfide production |
25453414 | Argininosuccinate synthase | 2 | 291.93 | 17.23 | 10 | 46.5 | 7.78 | Alanine, aspartate and glutamate metabolism; Arginine biosynthesis; Biosynthesis of amino acids; Urea cycle |
1374715 | ATP synthase β subunit | 3 | 360.79 | 8.42 | 4 | 51.2 | 5.02 | Oxidative phosphorylation; Metabolic pathways |
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Zhang, T.; Wei, W.; Dirsch, O.; Krüger, T.; Kan, C.; Xie, C.; Kniemeyer, O.; Fang, H.; Settmacher, U.; Dahmen, U. Identification of Proteins Interacting with Cytoplasmic High-Mobility Group Box 1 during the Hepatocellular Response to Ischemia Reperfusion Injury. Int. J. Mol. Sci. 2017, 18, 167. https://doi.org/10.3390/ijms18010167
Zhang T, Wei W, Dirsch O, Krüger T, Kan C, Xie C, Kniemeyer O, Fang H, Settmacher U, Dahmen U. Identification of Proteins Interacting with Cytoplasmic High-Mobility Group Box 1 during the Hepatocellular Response to Ischemia Reperfusion Injury. International Journal of Molecular Sciences. 2017; 18(1):167. https://doi.org/10.3390/ijms18010167
Chicago/Turabian StyleZhang, Tianjiao, Weiwei Wei, Olaf Dirsch, Thomas Krüger, Chunyi Kan, Chichi Xie, Olaf Kniemeyer, Haoshu Fang, Utz Settmacher, and Uta Dahmen. 2017. "Identification of Proteins Interacting with Cytoplasmic High-Mobility Group Box 1 during the Hepatocellular Response to Ischemia Reperfusion Injury" International Journal of Molecular Sciences 18, no. 1: 167. https://doi.org/10.3390/ijms18010167