A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline
AbstractIn vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell line-dependent, resulting in a minimal stimulation index of five followed by a rapid return to baseline insulin secretion by reducing glucose concentrations after a first high glucose stimulation. This protocol solves issues concerning in vitro research for type I diabetes as donors and the availability of primary islets of Langerhans are limited. To avoid the limitations of using human donor material, cell lines represent a valid alternative. Many different β cell lines have been reported, but the lack of reproducible responsiveness to glucose stimulation remains a challenge. View Full-Text
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Groot Nibbelink, M.; Marchioli, G.; Moroni, L.; Karperien, M.; Van Apeldoorn, A. A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline. Int. J. Mol. Sci. 2016, 17, 1532.
Groot Nibbelink M, Marchioli G, Moroni L, Karperien M, Van Apeldoorn A. A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline. International Journal of Molecular Sciences. 2016; 17(9):1532.Chicago/Turabian Style
Groot Nibbelink, Milou; Marchioli, Giulia; Moroni, Lorenzo; Karperien, Marcel; Van Apeldoorn, Aart. 2016. "A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline." Int. J. Mol. Sci. 17, no. 9: 1532.
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