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Article

Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes)

by
Lin Cong
1,†,
Fei Chen
2,†,
Shijiang Yu
1,
Lili Ding
1,
Juan Yang
1,
Ren Luo
1,
Huixia Tian
1,
Hongjun Li
1,
Haoqiang Liu
1 and
Chun Ran
1,*
1
Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, China
2
Sinofert Holdings Limited, Henan Branch, Zhengzhou 450000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this study.
Int. J. Mol. Sci. 2016, 17(6), 704; https://doi.org/10.3390/ijms17060704
Submission received: 25 March 2016 / Revised: 29 April 2016 / Accepted: 3 May 2016 / Published: 27 May 2016
(This article belongs to the Section Biochemistry)
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Abstract

Several fenpropathrin-resistant predatory mites have been reported. However, the molecular mechanism of the resistance remains unknown. In the present study, the Neoseiulus barkeri (N. barkeri) transcriptome was generated using the Illumina sequencing platform, 34,211 unigenes were obtained, and 15,987 were manually annotated. After manual annotation, attentions were attracted to resistance-related genes, such as voltage-gated sodium channel (VGSC), cytochrome P450s (P450s), and glutathione S-transferases (GSTs). A polymorphism analysis detected two point mutations (E1233G and S1282G) in the linker region between VGSC domain II and III. In addition, 43 putative P450 genes and 10 putative GST genes were identified from the transcriptome. Among them, two P450 genes, NbCYP4EV2 and NbCYP4EZ1, and four GST genes, NbGSTd01, NbGSTd02, NbGSTd03 and NbGSTm03, were remarkably overexpressed 3.64–46.69-fold in the fenpropathrin resistant strain compared to that in the susceptible strain. These results suggest that fenpropathrin resistance in N. barkeri is a complex biological process involving many genetic changes and provide new insight into the N. barkeri resistance mechanism.
Keywords: Neoseiulus barkeri; transcriptome; fenpropathrin resistant; VGSC; P450; GST Neoseiulus barkeri; transcriptome; fenpropathrin resistant; VGSC; P450; GST
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MDPI and ACS Style

Cong, L.; Chen, F.; Yu, S.; Ding, L.; Yang, J.; Luo, R.; Tian, H.; Li, H.; Liu, H.; Ran, C. Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes). Int. J. Mol. Sci. 2016, 17, 704. https://doi.org/10.3390/ijms17060704

AMA Style

Cong L, Chen F, Yu S, Ding L, Yang J, Luo R, Tian H, Li H, Liu H, Ran C. Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes). International Journal of Molecular Sciences. 2016; 17(6):704. https://doi.org/10.3390/ijms17060704

Chicago/Turabian Style

Cong, Lin, Fei Chen, Shijiang Yu, Lili Ding, Juan Yang, Ren Luo, Huixia Tian, Hongjun Li, Haoqiang Liu, and Chun Ran. 2016. "Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes)" International Journal of Molecular Sciences 17, no. 6: 704. https://doi.org/10.3390/ijms17060704

APA Style

Cong, L., Chen, F., Yu, S., Ding, L., Yang, J., Luo, R., Tian, H., Li, H., Liu, H., & Ran, C. (2016). Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes). International Journal of Molecular Sciences, 17(6), 704. https://doi.org/10.3390/ijms17060704

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