Next Article in Journal
Combining miRNA and mRNA Expression Profiles in Wilms Tumor Subtypes
Next Article in Special Issue
A Proteomic Approach for the Identification of Up-Regulated Proteins Involved in the Metabolic Process of the Leiomyoma
Previous Article in Journal
Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
Previous Article in Special Issue
Species Identification of Bovine, Ovine and Porcine Type 1 Collagen; Comparing Peptide Mass Fingerprinting and LC-Based Proteomics Methods
 
 
Article

The Expression Profile of Complement Components in Podocytes

Department of Pediatrics, Peking University First Hospital, Beijing 100034, China
*
Author to whom correspondence should be addressed.
Academic Editor: David Sheehan
Int. J. Mol. Sci. 2016, 17(4), 471; https://doi.org/10.3390/ijms17040471
Received: 29 January 2016 / Revised: 14 March 2016 / Accepted: 23 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Advances in Proteomic Research)
Podocytes are critical for maintaining the glomerular filtration barrier and are injured in many renal diseases, especially proteinuric kidney diseases. Recently, reports suggested that podocytes are among the renal cells that synthesize complement components that mediate glomerular diseases. Nevertheless, the profile and extent of complement component expression in podocytes remain unclear. This study examined the expression profile of complement in podocytes under physiological conditions and in abnormal podocytes induced by multiple stimuli. In total, 23/32 complement component components were detected in podocyte by conventional RT-PCR. Both primary cultured podocytes and immortalized podocytes expressed the complement factors C1q, C1r, C2, C3, C7, MASP, CFI, DAF, CD59, C4bp, CD46, Protein S, CR2, C1qR, C3aR, C5aR, and Crry (17/32), whereas C4, CFB, CFD, C5, C6, C8, C9, MBL1, and MBL2 (9/32) complement factors were not expressed. C3, Crry, and C1q-binding protein were detected by tandem mass spectrometry. Podocyte complement gene expression was affected by several factors (puromycin aminonucleoside (PAN), angiotensin II (Ang II), interleukin-6 (IL-6), and transforming growth factor-β (TGF-β)). Representative complement components were detected using fluorescence confocal microscopy. In conclusion, primary podocytes express various complement components at the mRNA and protein levels. The complement gene expressions were affected by several podocyte injury factors. View Full-Text
Keywords: podocyte; complement expression; podocyte injury podocyte; complement expression; podocyte injury
Show Figures

Graphical abstract

MDPI and ACS Style

Li, X.; Ding, F.; Zhang, X.; Li, B.; Ding, J. The Expression Profile of Complement Components in Podocytes. Int. J. Mol. Sci. 2016, 17, 471. https://doi.org/10.3390/ijms17040471

AMA Style

Li X, Ding F, Zhang X, Li B, Ding J. The Expression Profile of Complement Components in Podocytes. International Journal of Molecular Sciences. 2016; 17(4):471. https://doi.org/10.3390/ijms17040471

Chicago/Turabian Style

Li, Xuejuan, Fangrui Ding, Xiaoyan Zhang, Baihong Li, and Jie Ding. 2016. "The Expression Profile of Complement Components in Podocytes" International Journal of Molecular Sciences 17, no. 4: 471. https://doi.org/10.3390/ijms17040471

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop