Identification of Powdery Mildew Responsive Genes in Hevea brasiliensis through mRNA Differential Display
by
Xiang Li 1
, Zhenghong Bi 2, Rong Di 1,3, Peng Liang 1, Qiguang He 1, Wenbo Liu 1, Weiguo Miao 1,* and Fucong Zheng 1,*
Xiang Li 1, Zhenghong Bi 2, Rong Di 1,3, Peng Liang 1, Qiguang He 1, Wenbo Liu 1, Weiguo Miao 1,* and Fucong Zheng 1,*
1
College of Environment and Plant Protection, Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Hainan University, No. 58, Renmin Avenue, Haikou 570228, China
2
College of Agriculture, Hainan University, Haikou 570228, China
3
Department of Plant Biology and Pathology, Rutgers, the State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Int. J. Mol. Sci. 2016, 17(2), 181; https://doi.org/10.3390/ijms17020181
Received: 5 December 2015 / Revised: 21 January 2016 / Accepted: 22 January 2016 / Published: 29 January 2016
(This article belongs to the Special Issue Plant Molecular Biology)
Powdery mildew is an important disease of rubber trees caused by Oidium heveae B. A. Steinmann. As far as we know, none of the resistance genes related to powdery mildew have been isolated from the rubber tree. There is little information available at the molecular level regarding how a rubber tree develops defense mechanisms against this pathogen. We have studied rubber tree mRNA transcripts from the resistant RRIC52 cultivar by differential display analysis. Leaves inoculated with the spores of O. heveae were collected from 0 to 120 hpi in order to identify pathogen-regulated genes at different infection stages. We identified 78 rubber tree genes that were differentially expressed during the plant–pathogen interaction. BLAST analysis for these 78 ESTs classified them into seven functional groups: cell wall and membrane pathways, transcription factor and regulatory proteins, transporters, signal transduction, phytoalexin biosynthesis, other metabolism functions, and unknown functions. The gene expression for eight of these genes was validated by qRT-PCR in both RRIC52 and the partially susceptible Reyan 7-33-97 cultivars, revealing the similar or differential changes of gene expressions between these two cultivars. This study has improved our overall understanding of the molecular mechanisms of rubber tree resistance to powdery mildew.
View Full-Text
Keywords:
rubber tree; Oidium heveae; differential gene expression; ESTs
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Li, X.; Bi, Z.; Di, R.; Liang, P.; He, Q.; Liu, W.; Miao, W.; Zheng, F. Identification of Powdery Mildew Responsive Genes in Hevea brasiliensis through mRNA Differential Display. Int. J. Mol. Sci. 2016, 17, 181.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
- Supplementary File 1:
Supplementary (PDF, 701 KB)
