Next Article in Journal
Methyl Sartortuoate Inhibits Colon Cancer Cell Growth by Inducing Apoptosis and G2/M-Phase Arrest
Previous Article in Journal
DNA Targeting Sequence Improves Magnetic Nanoparticle-Based Plasmid DNA Transfection Efficiency in Model Neurons
Previous Article in Special Issue
Soy and Breast Cancer: Focus on Angiogenesis
Open AccessArticle

15,16-Dihydrotanshinone I from the Functional Food Salvia miltiorrhiza Exhibits Anticancer Activity in Human HL-60 Leukemia Cells: in Vitro and in Vivo Studies

1
School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No. 250 Wuxing St., Taipei 11031, Taiwan
2
Department of Radiology and Nuclear Medicine, Yuan’s General Hospital, No. 162 Cheng Kung 1st Road, Kaohsiung 80249, Taiwan
3
Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, No. 252 Wuxing St., Taipei 11031, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editor: Sanjay K. Srivastava
Int. J. Mol. Sci. 2015, 16(8), 19387-19400; https://doi.org/10.3390/ijms160819387
Received: 31 March 2015 / Revised: 3 August 2015 / Accepted: 6 August 2015 / Published: 17 August 2015
(This article belongs to the Special Issue Bioactive Phytochemicals in Functional Foods for Cancer Prevention)
15,16-Dihydrotanshinone I (DHTS) is extracted from Salvia miltiorrhiza Bunge which is a functional food in Asia. In this study, we investigated the apoptotic effect of DHTS on the human acute myeloid leukemia (AML) type III HL-60 cell line. We found that treatment with 1.5 μg/mL DHTS increased proapoptotic Bax and Bad protein expressions and activated caspases-3, -8, and -9, thus leading to poly ADP ribose polymerase (PARP) cleavage and resulting in cell apoptosis. DHTS induced sustained c-Jun N-terminal kinase (JNK) phosphorylation and Fas ligand (FasL) expression. The anti-Fas blocking antibody reversed the DHTS-induced cell death, and the JNK-specific inhibitor, SP600125, inhibited DHTS-induced caspase-3, -8, -9, and PARP cleavage. In a xenograft nude mice model, 25 mg/kg DHTS showed a great effect in attenuating HL-60 tumor growth. Taken together, these results suggest that DHTS can induce HL-60 cell apoptosis in vitro and inhibit HL-60 cell growth in vivo; the underlying mechanisms might be mediated through activation of the JNK and FasL signal pathways. View Full-Text
Keywords: 15,16-dihydrotanshinone I; acute myeloid leukemia; c-Jun N-terminal kinase; Fas ligand; apoptosis 15,16-dihydrotanshinone I; acute myeloid leukemia; c-Jun N-terminal kinase; Fas ligand; apoptosis
Show Figures

Figure 1

MDPI and ACS Style

Liu, J.-J.; Wu, H.-H.; Chen, T.-H.; Leung, W.; Liang, Y.-C. 15,16-Dihydrotanshinone I from the Functional Food Salvia miltiorrhiza Exhibits Anticancer Activity in Human HL-60 Leukemia Cells: in Vitro and in Vivo Studies. Int. J. Mol. Sci. 2015, 16, 19387-19400. https://doi.org/10.3390/ijms160819387

AMA Style

Liu J-J, Wu H-H, Chen T-H, Leung W, Liang Y-C. 15,16-Dihydrotanshinone I from the Functional Food Salvia miltiorrhiza Exhibits Anticancer Activity in Human HL-60 Leukemia Cells: in Vitro and in Vivo Studies. International Journal of Molecular Sciences. 2015; 16(8):19387-19400. https://doi.org/10.3390/ijms160819387

Chicago/Turabian Style

Liu, Jun-Jen; Wu, Hsueh-Hsia; Chen, Tzu-Ho; Leung, Wan; Liang, Yu-Chih. 2015. "15,16-Dihydrotanshinone I from the Functional Food Salvia miltiorrhiza Exhibits Anticancer Activity in Human HL-60 Leukemia Cells: in Vitro and in Vivo Studies" Int. J. Mol. Sci. 16, no. 8: 19387-19400. https://doi.org/10.3390/ijms160819387

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop