Next Article in Journal
Scanning for Therapeutic Targets within the Cytokine Network of Idiopathic Inflammatory Myopathies
Next Article in Special Issue
BMP9-Induced Survival Effect in Liver Tumor Cells Requires p38MAPK Activation
Previous Article in Journal
Bioactive Compounds of Blueberries: Post-Harvest Factors Influencing the Nutritional Value of Products
Previous Article in Special Issue
1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(8), 18664-18682;

Changes of the Cytoplasmic Proteome in Response to Alcoholic Hepatotoxicity in Rats

College of Interdisciplinary & Creative Studies, Konyang University, Nonsan 320-711, Korea
College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea
Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu 702-701, Korea
College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Johannes Haybaeck
Received: 9 June 2015 / Revised: 1 August 2015 / Accepted: 4 August 2015 / Published: 10 August 2015
(This article belongs to the Collection Molecular Mechanisms of Human Liver Diseases)
Full-Text   |   PDF [2529 KB, uploaded 10 August 2015]   |  


Proteomic analyses have already been used in a number of hepatological studies and provide important information. However, few reports have focused on changes in the cytoplasmic proteome. The present study therefore aimed to evaluate changes in cytoplasmic proteome of rats in response to alcoholic hepatotoxicity. Rats were fed a Liber-DeCarli liquid diet containing ethanol for four weeks. Cytoplasmic proteins except mitochondrial proteins from the livers of these animals were investigated using two-dimensional gel electrophoresis and mass spectrometry. Alcohol induced a decrease in body weight gain and an increase in alanine transaminase (ALT), cholesterol, and phospholipid levels. Histopathological observations revealed hepatic damage characterized by necrosis and fatty change in alcohol-treated group at week 2, which continues until week 4. Our proteomic analysis revealed that 25 proteins were differentially expressed in the ethanol-fed group. Of these, 12 cytoplasmic proteins are being reported for the first time. Taken together, our results provide further insights into the disease mechanism and therapeutic information of alcoholic liver disease. View Full-Text
Keywords: alcohol; hepatotoxicity; proteomics; cytoplasm alcohol; hepatotoxicity; proteomics; cytoplasm

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Kim, D.H.; Lee, E.-M.; Do, S.-H.; Jeong, D.-H.; Jeong, K.-S. Changes of the Cytoplasmic Proteome in Response to Alcoholic Hepatotoxicity in Rats. Int. J. Mol. Sci. 2015, 16, 18664-18682.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top