A New Ligustrazine Derivative-Selective Cytotoxicity by Suppression of NF-κB/p65 and COX-2 Expression on Human Hepatoma Cells. Part 3
Abstract
:1. Introduction
2. Results and Discussion
2.1. In Vitro Cytotoxicity
Compound | IC50 Values (µM) | ||||
---|---|---|---|---|---|
Bel-7402 | HeLa | HT-29 | BGC-823 | MDCK | |
T-OA | 6.36 ± 1.56 | 28.73 ± 5.89 | 29.84 ± 6.73 | >30 | >150 |
Cisplatin | 5.94 ± 1.48 | 6.22 ± 2.06 | 6.11 ± 2.31 | 6.82 ± 1.79 | 3.70 ± 2.89 |
2.2. Giemsa Staining
2.3. DAPI Staining
2.4. AO/EB Staining
2.5. Apoptosis Analysis
2.6. Mitochondrial Membrane Potential Assay
2.7. Immumohistochemical Staining
2.8. Subcellular Localization
3. Experimental Section
3.1. General
3.2. Cytotoxicity Evaluation
3.3. Morphological Examination by Giemsa Staining
3.4. DAPI Staining
3.5. AO/EB Staining
3.6. Apoptosis Analysis
3.7. Mitochondrial Membrane Potential Assay
3.8. Immumohistochemical Staining
3.9. Subcellular Localization of T-OA
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhang, C.; Yan, W.; Li, B.; Xu, B.; Gong, Y.; Chu, F.; Zhang, Y.; Yao, Q.; Wang, P.; Lei, H. A New Ligustrazine Derivative-Selective Cytotoxicity by Suppression of NF-κB/p65 and COX-2 Expression on Human Hepatoma Cells. Part 3. Int. J. Mol. Sci. 2015, 16, 16401-16413. https://doi.org/10.3390/ijms160716401
Zhang C, Yan W, Li B, Xu B, Gong Y, Chu F, Zhang Y, Yao Q, Wang P, Lei H. A New Ligustrazine Derivative-Selective Cytotoxicity by Suppression of NF-κB/p65 and COX-2 Expression on Human Hepatoma Cells. Part 3. International Journal of Molecular Sciences. 2015; 16(7):16401-16413. https://doi.org/10.3390/ijms160716401
Chicago/Turabian StyleZhang, Chenze, Wenqiang Yan, Bi Li, Bing Xu, Yan Gong, Fuhao Chu, Yuzhong Zhang, Qiuli Yao, Penglong Wang, and Haimin Lei. 2015. "A New Ligustrazine Derivative-Selective Cytotoxicity by Suppression of NF-κB/p65 and COX-2 Expression on Human Hepatoma Cells. Part 3" International Journal of Molecular Sciences 16, no. 7: 16401-16413. https://doi.org/10.3390/ijms160716401