Stress-Responsive Expression, Subcellular Localization and Protein–Protein Interactions of the Rice Metacaspase Family
Abstract
:1. Introduction
2. Results
2.1. Expression Patterns of OsMCs in Response to Abiotic Stresses
2.1.1. Expression Patterns in Response to Drought and Salt Stresses
2.1.2. Expression Patterns in Response to Cold and Heat Stresses
2.2. Expression Patterns of OsMCs in Response to Pathogens
2.2.1. Expression Patterns in Response to M. oryzae
2.2.2. Expression Patterns in Response to Xoo
2.2.3. Expression Patterns in Response to R. solani
2.3. Expression Patterns of OsMCs in Response to Stress-Related Hormones
2.4. Putative Cis-Elements in Promoter Regions of the OsMC Genes
Regulator | Cis-Element | Code | Numbers of cis-Elements | |||||||
---|---|---|---|---|---|---|---|---|---|---|
OsMC1 | OsMC2 | OsMC3 | OsMC4 | OsMC5 | OsMC6 | OsMC7 | OsMC8 | |||
ABA | ABRELATERD1 | S000414 | – | 2 | 1 | – | – | 2 | – | 1 |
ABREOSRAB21 | S000012 | – | – | 2 | – | – | – | – | – | |
ABRERATCAL | S000408 | – | 3 | – | – | – | 2 | – | 1 | |
MYB | MYBCORE | S000176 | 2 | 4 | 5 | 1 | 1 | 2 | 1 | 1 |
MYC | MYCCONSENSUSAT | S000407 | 4 | 14 | 8 | 4 | 4 | 8 | 6 | 8 |
WRKY | WBOXATNPR1 | S000390 | 1 | 1 | 1 | – | 4 | – | – | 3 |
WBOXNTERF3 | S000457 | 2 | 1 | 1 | 1 | 2 | – | 1 | 6 | |
WRKY710S | S000447 | 3 | 4 | 3 | 4 | 6 | 1 | 1 | 10 | |
ERF | GCCCORE | S000430 | – | – | 3 | 1 | 2 | 2 | – | 1 |
Auxin | NTBBF1ARROLB | S000273 | 1 | – | – | – | 1 | – | – | – |
2.5. Subcellular Localization of OsMCs
2.6. Interaction Relationships between Type I OsMCs and OsLSDs
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth
4.2. Treatments with Abiotic Stress and Hormones
4.3. Inoculation with Different Pathogens
4.4. qRT-PCR Analysis of Gene Expression
Primers | Sequences (5′-3′) | Size (bp) |
---|---|---|
Cloning of cDNAs | ||
OsMC1-F | ATGGATCACTTCGGCGGACG | 1107 |
OsMC1-R | TTACAGGACGAACGGCTTGC | |
OsMC2-F | ATGGCGAGCGCGAGGCCGCC | 1110 |
OsMC2-R | TCACAAGAGGAAGGGCTTCC | |
OsMC3-F | ATGGGCTGCAACTGCCTCGT | 1203 |
OsMC3-R | TCACAGGAGAAACGGTTTCC | |
OsMC4-F | ATGGGGCGGAAGAGAGCGGT | 1230 |
OsMC4-R | TTAGCATATGAAAGCCACGT | |
OsMC5-F | ATGGGGGGCCGGAAGCGCGC | 1263 |
OsMC5-R | TCAGCATATGAAGGCCACAC | |
OsMC6-F | ATGGGCCGCAAGCGCGCGCT | 1254 |
OsMC6-R | TCAGCATATAAAAGACACAT | |
OsMC7-F | ATGGAGAGGGGTCAGAAGAA | 1026 |
OsMC7-R | TCAGAGCGCCGTCATGGCCT | |
OsMC8-F | ATGGCGGTCGTCAGCGGCGG | 909 |
OsMC8-R | TCACAGGATAAACTGCTCCT | |
OsLSD1-F | ATGTGCATTGCTGAACCAAT | 606 |
OsLSD1-R | TCAGCTGCTGGGCTTCTGGT | |
OsLSD2-F | ATGGTGGCTTCAAGAGCTCCA | 444 |
OsLSD2-R | CTATCCTAGACTGAAAAGCA | |
OsLSD3-F | ATGCAGAGCCAGATCGTGT | 519 |
OsLSD3-R | TTACTTTTTACCACCAGTTGTA | |
OsLSD4-F | ATGCAGAGCCAGATCGTGTG | 561 |
OsLSD4-R | CTATTTCCCAGTTGTAACTCCA | |
OsLSD5-F | ATGCAGGACCAGCTGATCTG | 444 |
OsLSD5-R | TCATCTTTTCCATGAGGTGAC | |
Subcellular Localization Assays | ||
OsMC1-GFP-F | CGCGGATCC ATGGATCACTTCGGCGGACG | 1107 |
OsMC1-GFP-R | TGCTCTAGA TTACAGGACGAACGGCTTGC | |
OsMC2-GFP-F | CGCGGATCC ATGGCGAGCGCGAGGCCGCC | 1110 |
OsMC2-GFP-R | TGCTCTAGA TCACAAGAGGAAGGGCTTCC | |
OsMC3-GFP-F | CGCGGATCC ATGGGCTGCAACTGCCTCGT | 1203 |
OsMC3-GFP-R | TGCTCTAGA TCACAGGAGAAACGGTTTCC | |
OsMC4-GFP-F | CGCGGATCC ATGGGGCGGAAGAGAGCGGT | 1230 |
OsMC4-GFP-R | TGCTCTAGA TTAGCATATGAAAGCCACGT | |
OsMC5-GFP-F | CGCGGATCC ATGGGGGGCCGGAAGCGCGC | 1263 |
OsMC5-GFP-R | TGCTCTAGA TCAGCATATGAAGGCCACAC | |
OsMC6-GFP-F | CGCGGATCC ATGGGCCGCAAGCGCGCGCT | 1254 |
OsMC6-GFP-R | TGCTCTAGA TCAGCATATAAAAGACACAT | |
OsMC7-GFP-F | CGCGGATCC ATGGAGAGGGGTCAGAAGAA | 1026 |
OsMC7-GFP-R | TGCTCTAGA TCAGAGCGCCGTCATGGCCT | |
OsMC8-GFP-F | CGCGGATCC ATGGCGGTCGTCAGCGGCGG | 909 |
OsMC8-GFP-R | TCCCCCGGG TCACAGGATAAACTGCTCCT | |
qRT-PCR Assays | ||
OsMC1-RT-F | GCTTCATCAAGGCGGTGGAGTG | 142 |
OsMC1-RT-R | AAGTTGGCGACCTTGCGGATG | |
OsMC2-RT-F | CGACCCGTACAGGGTGCCGA | 166 |
OsMC2-RT-R | GCACAGCGCCTCGTCGTAGC | |
OsMC3-RT-F | GGCTCCTTCGTCCGCAAGAT | 101 |
OsMC3-RT-R | CACAGGAGAAACGGTTTCCTGT | |
OsMC4-RT-F | TCGACGTTCGTGGAGATGCTC | 126 |
OsMC4-RT-R | ATTCACGAGCCGCCTGATCTT | |
OsMC5-RT-F | GTGCCAGACCGACCAGACAT | 102 |
OsMC5-RT-R | CCGCTCTTCTCCGACAGGAT | |
OsMC1-RT-F | GCTTCATCAAGGCGGTGGAGTG | 142 |
OsMC1-RT-R | AAGTTGGCGACCTTGCGGATG | |
OsMC6-RT-F | CCACACCGCAGGGTTCTTCAT | 147 |
OsMC6-RT-R | GTCCAGGCTGCTGAGTGTATCC | |
OsMC7-RT-F | ATACAGACCGTGCTGGCGTC | 143 |
OsMC7-RT-R | AGGAATGGCGTCTCGGCGTT | |
OsMC8-RT-F | TCCGGCAAGTGCCTCGTAAC | 150 |
OsMC8-RT-R | CAATGCGGTCGGTCACAGGAT | |
Yeast Two-Hybrid Assays | ||
OsMC1-BD-F | CGGAATTC ATGGATCACTTCGGCGGACGT | 1107 |
OsMC1-BD-R | CGCGGATCC TTACAGGACGAACGGCTTGCG | |
OsMC2-BD-F | CCGGAATTC ATGGCGAGCGCGAGGCCG | 1110 |
OsMC2-BD-R | CGCGGATCC TCACAAGAGGAAGGGCTTC | |
OsMC3-BD-F | CCGGAATTC ATGGGCTGCAACTGCCTCGTC | 1203 |
OsMC3-BD-R | CGCGGATCC TCACAGGAGAAACGGTTTCC | |
OsMC1-BDN-R | TGCTCTAGA CTTGCCGCGGGAGCCCGGGA | 252 |
OsMC1-BDC-F | CGCGGATCC AAGCGCGCCGTCCTGATCGGC | 855 |
OsLSD1-AD-F | CCGGAATTC ATGTGCATTGCTGAACCAAT | 606 |
OsLSD1-AD-R | CGCGGATCC TCAGCTGCTGGGCTTCTGGT | |
OsLSD2-AD-F | CCGGAATTC ATGGTGGCTTCAAGAGCTCCA | 444 |
OsLSD2-AD-R | CGCGGATCC CTATCCTAGACTGAAAAGCA | |
OsLSD3-AD-F | CCGGAATTC ATGCAGAGCCAGATCGTGT | 519 |
OsLSD3-AD-R | CGCGGATCC TTACTTTTTACCACCAGTTGTA | |
OsLSD4-AD-F | CCGGAATTC ATGCAGAGCCAGATCGTGTG | 561 |
OsLSD4-AD-R | CGCGGATCC CTATTTCCCAGTTGTAACTCCA | |
OsLSD5-AD-F | CCGGAATTC ATGCAGGACCAGCTGATCTG | 444 |
OsLSD5-AD-R | CGCGGATCC TCATCTTTTCCATGAGGTGAC |
4.5. Subcellular Localization of the OsMC Proteins
4.6. Yeast Two-Hybrid Interaction Assays
4.7. Bioinformatics Analysis of Cis-Element in the Promoters of the OsMC Genes
4.8. Accession Numbers
5. Conclusions
Genes | M. oryzae | Xoo | R. solani | Abiotic Stress | Hormones | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Compatible | Incompatible | Compatible | Incompatible | Drought | Salt | Heat | Cold | ACC | JA | SA | ABA | ||
OsMC1 | ↓ | ↑ | ↓ | -- | ↑ | ↓ | ↑ | ↑ | -- | ↑ | ↓ | ↑ | ↑ |
OsMC2 | -- | -- | -- | ↑ | -- | ↓ | -- | -- | -- | ↓ | -- | ↓ | ↑ |
OsMC3 | ↓ | ↑ | -- | -- | ↓ | -- | -- | ↑ | ↓ | -- | -- | -- | -- |
OsMC4 | ↓ | -- | ↑ | -- | -- | ↓ | ↓ | ↓ | ↑ | ↓ | -- | ↑ | ↓ |
OsMC5 | ↑ | ↑ | -- | -- | ↓ | -- | ↓ | ↑ | ↓ | -- | -- | -- | ↓ |
OsMC6 | -- | ↑ | -- | -- | ↓ | ↓ | ↓ | ↑ | -- | ↑ | -- | -- | ↓ |
OsMC7 | ↑ | ↑ | ↓ | -- | ↑ | ↑ | ↑ | ↓ | ↓ | -- | ↓ | ↓ | ↑ |
OsMC8 | ↓ | -- | -- | ↑ | ↓ | -- | ↓ | -- | -- | -- | -- | ↓ | -- |
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Huang, L.; Zhang, H.; Hong, Y.; Liu, S.; Li, D.; Song, F. Stress-Responsive Expression, Subcellular Localization and Protein–Protein Interactions of the Rice Metacaspase Family. Int. J. Mol. Sci. 2015, 16, 16216-16241. https://doi.org/10.3390/ijms160716216
Huang L, Zhang H, Hong Y, Liu S, Li D, Song F. Stress-Responsive Expression, Subcellular Localization and Protein–Protein Interactions of the Rice Metacaspase Family. International Journal of Molecular Sciences. 2015; 16(7):16216-16241. https://doi.org/10.3390/ijms160716216
Chicago/Turabian StyleHuang, Lei, Huijuan Zhang, Yongbo Hong, Shixia Liu, Dayong Li, and Fengming Song. 2015. "Stress-Responsive Expression, Subcellular Localization and Protein–Protein Interactions of the Rice Metacaspase Family" International Journal of Molecular Sciences 16, no. 7: 16216-16241. https://doi.org/10.3390/ijms160716216