DDX3X Biomarker Correlates with Poor Survival in Human Gliomas
Abstract
:1. Introduction
2. Result
2.1. DDX3X Expression Positively Correlates with WHO Grading of Human Gliomas
2.2. DDX3X Expression Correlates with Poor Survival in High-Grade Gliomas
2.3. DDX3X mRNA Expression Is Increased in Human Glioma Cells
2.4. DDX3X Protein Overexpresses in Human Glioma Cells
2.5. DDX3X Protein Production Overexpressed in Human High-Grade Gliomas
2.6. DDX3X Hub Protein and the Protein–Protein Interactions
3. Discussion
4. Materials and Methods
4.1. Study Ethics and DDX3X Gene Expression in Human Brain Tumor
4.2. RNA Isolation and Quantitative RT-PCR (qRT-PCR)
4.3. Cell Lysate Preparation and Western Blots
4.4. Immunohistochemical (IHC) Staining
4.5. Evaluation of DDX3X Immunostain Scores
4.6. PPI Network and Signaling Pathways Analysis
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hueng, D.-Y.; Tsai, W.-C.; Chiou, H.-Y.C.; Feng, S.-W.; Lin, C.; Li, Y.-F.; Huang, L.-C.; Lin, M.-H. DDX3X Biomarker Correlates with Poor Survival in Human Gliomas. Int. J. Mol. Sci. 2015, 16, 15578-15591. https://doi.org/10.3390/ijms160715578
Hueng D-Y, Tsai W-C, Chiou H-YC, Feng S-W, Lin C, Li Y-F, Huang L-C, Lin M-H. DDX3X Biomarker Correlates with Poor Survival in Human Gliomas. International Journal of Molecular Sciences. 2015; 16(7):15578-15591. https://doi.org/10.3390/ijms160715578
Chicago/Turabian StyleHueng, Dueng-Yuan, Wen-Chiuan Tsai, Hsin-Ying Clair Chiou, Shao-Wei Feng, Chin Lin, Yao-Feng Li, Li-Chun Huang, and Ming-Hong Lin. 2015. "DDX3X Biomarker Correlates with Poor Survival in Human Gliomas" International Journal of Molecular Sciences 16, no. 7: 15578-15591. https://doi.org/10.3390/ijms160715578