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Article

Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm

by
Rafaelle Spear
1,†,
Ludovic Boytard
1,†,
Renaud Blervaque
2,
Maggy Chwastyniak
1,
David Hot
2,
Jonathan Vanhoutte
3,
Bart Staels
3,
Yves Lemoine
2,
Nicolas Lamblin
1,
François-René Pruvot
4,
Stephan Haulon
5,
Philippe Amouyel
1 and
Florence Pinet
1,*
1
National Institute of Health and Medical Research (INSERM) U1167, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France
2
INSERM U1019, National Center of Scientific Research (CNRS) Join Research Unit (UMR) 8204, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France
3
INSERM U1011, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France
4
Lille Regional University Hospital, F-59037 Lille, France
5
INSERM U1008, Lille North of France University, Lille Regional University Hospital, F-59000 Lille, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2015, 16(5), 11276-11293; https://doi.org/10.3390/ijms160511276
Submission received: 30 October 2014 / Revised: 6 January 2015 / Accepted: 12 January 2015 / Published: 18 May 2015
(This article belongs to the Special Issue Advances in Peripheral Artery Disease)
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Abstract

Abdominal aortic aneurysm (AAA) is an inflammatory disease associated with marked changes in the cellular composition of the aortic wall. This study aims to identify microRNA (miRNA) expression in aneurysmal inflammatory cells isolated by laser microdissection from human tissue samples. The distribution of inflammatory cells (neutrophils, B and T lymphocytes, mast cells) was evaluated in human AAA biopsies. We observed in half of the samples that adventitial tertiary lymphoid organs (ATLOs) with a thickness from 0.5 to 2 mm were located exclusively in the adventitia. Out of the 850 miRNA that were screened by microarray in isolated ATLOs (n = 2), 164 miRNAs were detected in ATLOs. The three miRNAs (miR-15a-3p, miR-30a-5p and miR-489-3p) with the highest expression levels were chosen and their expression quantified by RT-PCR in isolated ATLOs (n = 4), M1 (n = 2) and M2 macrophages (n = 2) and entire aneurysmal biopsies (n = 3). Except for the miR-30a-5p, a similar modulation was found in ATLOs and the two subtypes of macrophages. The modulated miRNAs were then evaluated in the plasma of AAA patients for their potential as AAA biomarkers. Our data emphasize the potential of miR-15a-3p and miR-30a-5p as biomarkers of AAA but also as triggers of ATLO evolution. Further investigations will be required to evaluate their targets in order to better understand AAA pathophysiology.
Keywords: abdominal aortic aneurysm; adventitial tertiary lymphoid organs; microRNA; laser microdissection; quantitative RT-PCR abdominal aortic aneurysm; adventitial tertiary lymphoid organs; microRNA; laser microdissection; quantitative RT-PCR
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MDPI and ACS Style

Spear, R.; Boytard, L.; Blervaque, R.; Chwastyniak, M.; Hot, D.; Vanhoutte, J.; Staels, B.; Lemoine, Y.; Lamblin, N.; Pruvot, F.-R.; et al. Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm. Int. J. Mol. Sci. 2015, 16, 11276-11293. https://doi.org/10.3390/ijms160511276

AMA Style

Spear R, Boytard L, Blervaque R, Chwastyniak M, Hot D, Vanhoutte J, Staels B, Lemoine Y, Lamblin N, Pruvot F-R, et al. Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm. International Journal of Molecular Sciences. 2015; 16(5):11276-11293. https://doi.org/10.3390/ijms160511276

Chicago/Turabian Style

Spear, Rafaelle, Ludovic Boytard, Renaud Blervaque, Maggy Chwastyniak, David Hot, Jonathan Vanhoutte, Bart Staels, Yves Lemoine, Nicolas Lamblin, François-René Pruvot, and et al. 2015. "Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm" International Journal of Molecular Sciences 16, no. 5: 11276-11293. https://doi.org/10.3390/ijms160511276

APA Style

Spear, R., Boytard, L., Blervaque, R., Chwastyniak, M., Hot, D., Vanhoutte, J., Staels, B., Lemoine, Y., Lamblin, N., Pruvot, F.-R., Haulon, S., Amouyel, P., & Pinet, F. (2015). Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm. International Journal of Molecular Sciences, 16(5), 11276-11293. https://doi.org/10.3390/ijms160511276

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