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Article

Effect of the Characters of Chitosans Used and Regeneration Conditions on the Yield and Physicochemical Characteristics of Regenerated Products

by
Chu Hsi Hsu
1,2,
Szu Kai Chen
1,
Wei Yu Chen
1,
Min Lang Tsai
1,* and
Rong Huei Chen
1,*
1
Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, Taiwan
2
Department of Food and Beverage Management, Yuanpei University of Medical Technology, 306, Yuanpei Street, Hsinchu 30015, Taiwan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2015, 16(4), 8621-8634; https://doi.org/10.3390/ijms16048621
Submission received: 26 February 2015 / Revised: 25 March 2015 / Accepted: 9 April 2015 / Published: 17 April 2015
(This article belongs to the Special Issue Chitins 2015)
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Abstract

The objective of this study was to explore the effect of the character of chitosans used, and the regeneration conditions employed on, the yield and physicochemical characteristics of regenerated products. Different concentrations of acetic acid were used to dissolve chitosans of 61.7% and 94.9% degree of deacetylation (DD), and weight-average molecular weight (Mw) of 176 and 97 kDa, respectively; they were then precipitated with an 8 N NaOH solution, followed by washing and neutral and freeze drying to get the regenerated products. Yields of regenerated products and their physicochemical properties, such as ash content, bulk density, Mw, polydispersity index (PDI), DD, and crystallinity were measured. A higher concentration of acetic acid used resulted in a higher yield. The purity of the regenerated product increased significantly, whereas the bulk density and crystallinity decreased significantly after regeneration. The regeneration process showed its merits of narrowing down the PDI of regenerated products. The DD and structure of chitosan was changed insignificantly after the regeneration process.
Keywords: chitosan; regeneration; molecular weight; polydispersity index; degree of deacetylation; crystallinity; ash content chitosan; regeneration; molecular weight; polydispersity index; degree of deacetylation; crystallinity; ash content

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MDPI and ACS Style

Hsu, C.H.; Chen, S.K.; Chen, W.Y.; Tsai, M.L.; Chen, R.H. Effect of the Characters of Chitosans Used and Regeneration Conditions on the Yield and Physicochemical Characteristics of Regenerated Products. Int. J. Mol. Sci. 2015, 16, 8621-8634. https://doi.org/10.3390/ijms16048621

AMA Style

Hsu CH, Chen SK, Chen WY, Tsai ML, Chen RH. Effect of the Characters of Chitosans Used and Regeneration Conditions on the Yield and Physicochemical Characteristics of Regenerated Products. International Journal of Molecular Sciences. 2015; 16(4):8621-8634. https://doi.org/10.3390/ijms16048621

Chicago/Turabian Style

Hsu, Chu Hsi, Szu Kai Chen, Wei Yu Chen, Min Lang Tsai, and Rong Huei Chen. 2015. "Effect of the Characters of Chitosans Used and Regeneration Conditions on the Yield and Physicochemical Characteristics of Regenerated Products" International Journal of Molecular Sciences 16, no. 4: 8621-8634. https://doi.org/10.3390/ijms16048621

APA Style

Hsu, C. H., Chen, S. K., Chen, W. Y., Tsai, M. L., & Chen, R. H. (2015). Effect of the Characters of Chitosans Used and Regeneration Conditions on the Yield and Physicochemical Characteristics of Regenerated Products. International Journal of Molecular Sciences, 16(4), 8621-8634. https://doi.org/10.3390/ijms16048621

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