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Article

In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes

by
Rangarajulu Senthil Kumaran
1,*,
Yong-Keun Choi
1,
Vijay Singh
2,
Hak-Jin Song
1,
Kyung-Guen Song
3,
Kwang Jin Kim
4 and
Hyung Joo Kim
1
1
Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea
2
Department of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea
3
Water Environment Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang-Gu, Seoul 130650, Korea
4
Urban Agriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeonju 560500, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2015, 16(4), 7551-7564; https://doi.org/10.3390/ijms16047551
Submission received: 10 February 2015 / Revised: 19 March 2015 / Accepted: 30 March 2015 / Published: 3 April 2015
(This article belongs to the Special Issue Bioactive Nanoparticles)
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Abstract

Water-dispersible MgO nanoparticles were tested to investigate their cytotoxic effects on oxidative stress gene expression. In this in vitro study, genes related to reactive oxygen species (ROS), glutathione S-transferase (GST) and catalase, were quantified using real-time polymerase chain reactions (molecular level) and molecular beacon technologies (cellular level). The monodispersed MgO nanoparticles, 20 nm in size, were used to treat human cancer cell lines (liver cancer epithelial cells) at different concentrations (25, 75 and 150 µg/mL) and incubation times (24, 48 and 72 h). Both the genetic and cellular cytotoxic screening methods produced consistent results, showing that GST and catalase ROS gene expression was maximized at 150 µg/mL nanoparticle treatment with 48 h incubation. However, the genotoxic effect of MgO nanoparticles was not significant compared with control experiments, which indicates its significant potential applications in nanomedicine as a diagnostic and therapeutic tool.
Keywords: catalase; nano-genotoxicity; MgO nanoparticle; glutathione S-transferase; reactive oxygen species catalase; nano-genotoxicity; MgO nanoparticle; glutathione S-transferase; reactive oxygen species

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MDPI and ACS Style

Kumaran, R.S.; Choi, Y.-K.; Singh, V.; Song, H.-J.; Song, K.-G.; Kim, K.J.; Kim, H.J. In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes. Int. J. Mol. Sci. 2015, 16, 7551-7564. https://doi.org/10.3390/ijms16047551

AMA Style

Kumaran RS, Choi Y-K, Singh V, Song H-J, Song K-G, Kim KJ, Kim HJ. In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes. International Journal of Molecular Sciences. 2015; 16(4):7551-7564. https://doi.org/10.3390/ijms16047551

Chicago/Turabian Style

Kumaran, Rangarajulu Senthil, Yong-Keun Choi, Vijay Singh, Hak-Jin Song, Kyung-Guen Song, Kwang Jin Kim, and Hyung Joo Kim. 2015. "In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes" International Journal of Molecular Sciences 16, no. 4: 7551-7564. https://doi.org/10.3390/ijms16047551

APA Style

Kumaran, R. S., Choi, Y.-K., Singh, V., Song, H.-J., Song, K.-G., Kim, K. J., & Kim, H. J. (2015). In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes. International Journal of Molecular Sciences, 16(4), 7551-7564. https://doi.org/10.3390/ijms16047551

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