A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
Abstract
:1. Introduction
2. Results and Discussion
2.1. DPSC Isolation and Amplification
2.2. In Vitro Construction of a Dental Pulp-Like Tissue
2.3. In Vivo Experiments
3. Experimental Section
3.1. Biomaterial
3.2. DPSCs Isolation
3.3. Culture Media Preparation
3.4. DPSCs Differentiation in 3D
3.5. In Vitro Staining
3.6. Real-Time PCR
Gene Symbol | Forward Primer (5'→3') | Reverse Primer (5'→3') | Product Length (bp) |
---|---|---|---|
CNP | AACCTTCCTGTCTTCATCCTTAGC | ATCACAAGCCAACACACTCATTG | 137 |
COL1A1 | TGAGCCAGCAGATCGAGA | ACCAGTCTCCATGTTGCAGA | 178 |
GAPDH | TCAACAGCGACACCCAC | GGGTCTCTCTCTTCCTCTTGTG | 203 |
GFAP | AGGAAGATTGAGTCGCTGGAG | CGCCATTGCCTCATACTGC | 177 |
NES | TCAGAGGGAAGGAGATAGAGAGTC | AGCCAGAAACCATATGTCAAGAGA | 171 |
OCN | GCAGCGAGGTAGTGAAGAGAC | AGCAGAGCGACACCCTA | 193 |
ON | TGCATGTGTCTTAGTCTTAGTCACC | GCTAACTTAGTGCTTACAGGAACCA | 183 |
OPN | TGGAAAGCGAGGAGTTGAATGG | GCTCATTGCTCTCATCATTGGC | 192 |
PECAM1 | TCCAGCCAACTTCACCATCC | TGGGAGAGCATTTCACATACGA | 171 |
S100A4 | AGGGTGACAAGTTCAAGCTCAA | GCAGGACAGGAAGACACAGTA | 176 |
TUBB3 | GCTCTACGACATCTGCTTCCG | GAAGGGCACCATGTTGACG | 163 |
VWF | ACGTATGGTCTGTGTGGGATC | GACAAGACACTGCTCCTCCA | 159 |
Gene Symbol | Forward Primer (5'→3') | Reverse Primer (5'→3') | Product Length (bp) |
---|---|---|---|
BMP2 | TGCTCAGCTTCCATCACG | TTCCTGCATTTGTTCCCGAA | 152 |
BMP3 | GATAGCCACGTCAGAGAAGC | GCTTTCTCTCCTCCCACACTC | 139 |
COL1A1 | AAAGATGGCGAAGCTGGAG | GAAACCTCTCTCGCCTCTTG | 210 |
COL3A1 | AATGGTGACAGAGGAGAAACG | CCTCGATGTCCTTTGATGCC | 149 |
CTSB | TGTGGAGGTGTCTGCTGAG | GGAGGGATGGTGTAGGGTA | 168 |
CTSD | GTCGGTTCCATGTAAGTCAGA | ACTTGGCTGCGATGAATACG | 101 |
DSPP | AGCAGGCAACATCACAACC | TGACAGAGTAGATGAGTGGAGTG | 122 |
FGF8 | CATGGCAGAAGACGGAGAC | CCTTTGCCGTTGCTCTTGG | 147 |
GAPDH | GGCCTTCCGTGTTCCTA | AAGGTGGAAGAATGGGAGTTG | 192 |
MSX1 | GGGATGCAAAGGCGAAGA | GCTTTCTATAGGGCTGGGCTC | 186 |
TNFRSF11A | CAACTCAACGGATGGCTACA | TGGCTGCTGCTTCACTG | 134 |
3.7. In Vivo Experiments
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ferroni, L.; Gardin, C.; Sivolella, S.; Brunello, G.; Berengo, M.; Piattelli, A.; Bressan, E.; Zavan, B. A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue. Int. J. Mol. Sci. 2015, 16, 4666-4681. https://doi.org/10.3390/ijms16034666
Ferroni L, Gardin C, Sivolella S, Brunello G, Berengo M, Piattelli A, Bressan E, Zavan B. A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue. International Journal of Molecular Sciences. 2015; 16(3):4666-4681. https://doi.org/10.3390/ijms16034666
Chicago/Turabian StyleFerroni, Letizia, Chiara Gardin, Stefano Sivolella, Giulia Brunello, Mario Berengo, Adriano Piattelli, Eriberto Bressan, and Barbara Zavan. 2015. "A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue" International Journal of Molecular Sciences 16, no. 3: 4666-4681. https://doi.org/10.3390/ijms16034666