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Int. J. Mol. Sci. 2015, 16(12), 29936-29947;

Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells

The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
Author to whom correspondence should be addressed.
Academic Editor: Mateus Webba da Silva
Received: 3 November 2015 / Revised: 2 December 2015 / Accepted: 8 December 2015 / Published: 16 December 2015
(This article belongs to the Section Biochemistry)
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Tudor staphylococcal nuclease (Tudor-SN) is a highly conserved and ubiquitously expressed multifunctional protein, related to multiple and diverse cell type- and species-specific cellular processes. Studies have shown that Tudor-SN is mainly expressed in secretory cells, however knowledge of its role is limited. In our previous work, we found that the protein level of Tudor-SN was upregulated in the nucleus of bovine mammary epithelial cells (BMEC). In this study, we assessed the role of Tudor-SN in milk synthesis and cell proliferation of BMEC. We exploited gene overexpression and silencing methods, and found that Tudor-SN positively regulates milk synthesis and proliferation via Stat5a activation. Both amino acids (methionine) and estrogen triggered NFκB1 to bind to the gene promoters of Tudor-SN and Stat5a, and this enhanced the protein level and nuclear localization of Tudor-SN and p-Stat5a. Taken together, these results suggest the key role of Tudor-SN in the transcriptional regulation of milk synthesis and proliferation of BMEC under the stimulation of amino acids and hormones. View Full-Text
Keywords: Tudor-SN; milk synthesis; cell proliferation; Stat5; NFκB1 Tudor-SN; milk synthesis; cell proliferation; Stat5; NFκB1

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Ao, J.; Wei, C.; Si, Y.; Luo, C.; Lv, W.; Lin, Y.; Cui, Y.; Gao, X. Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells. Int. J. Mol. Sci. 2015, 16, 29936-29947.

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