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Article

Transcriptome Characterization for Non-Model Endangered Lycaenids, Protantigius superans and Spindasis takanosis, Using Illumina HiSeq 2500 Sequencing

1
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungcheongnam-do 31538, Korea
2
Trident School of Biotech Sciences, Trident Academy of Creative Technology (TACT), Chandaka Industrial Estate, Chandrasekharpur, Bhubaneswar, Odisha 751024, India
3
National Institute of Biological Resources, 42, Hwangyeong-ro, Seo-gu, Incheon 22689, Korea
4
Korea Zoonosis Research Institute (KOZRI), Chonbuk National University, 820-120 Hana-ro, Iksan, Jeollabuk-do 54528, Korea
5
Hampyeong County Insect Institute, Hampyeong County Agricultural Technology Center, 90, Hakgyohwasan-gil, Hakgyo-myeon, Hampyeong-gun, Jeollanan-do 57158, Korea
6
Research Institute, GnC BIO Co., LTD. 621-6 Banseok-dong, Yuseong-gu, Daejeon 34069, Korea
7
College of Agriculture and Life Science, Chonnam National University 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Lee A. Bulla
Int. J. Mol. Sci. 2015, 16(12), 29948-29970; https://doi.org/10.3390/ijms161226213
Received: 7 October 2015 / Revised: 28 November 2015 / Accepted: 9 December 2015 / Published: 16 December 2015
(This article belongs to the Section Biochemistry)
The Lycaenidae butterflies, Protantigius superans and Spindasis takanosis, are endangered insects in Korea known for their symbiotic association with ants. However, necessary genomic and transcriptomics data are lacking in these species, limiting conservation efforts. In this study, the P. superans and S. takanosis transcriptomes were deciphered using Illumina HiSeq 2500 sequencing. The P. superans and S. takanosis transcriptome data included a total of 254,340,693 and 245,110,582 clean reads assembled into 159,074 and 170,449 contigs and 107,950 and 121,140 unigenes, respectively. BLASTX hits (E-value of 1.0 × 10−5) against the known protein databases annotated a total of 46,754 and 51,908 transcripts for P. superans and S. takanosis. Approximately 41.25% and 38.68% of the unigenes for P. superans and S. takanosis found homologous sequences in Protostome DB (PANM-DB). BLAST2GO analysis confirmed 18,611 unigenes representing Gene Ontology (GO) terms and a total of 5259 unigenes assigned to 116 pathways for P. superans. For S. takanosis, a total of 6697 unigenes were assigned to 119 pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Additionally, 382,164 and 390,516 Simple Sequence Repeats (SSRs) were compiled from the unigenes of P. superans and S. takanosis, respectively. This is the first report to record new genes and their utilization for conservation of lycaenid species population and as a reference information for closely related species. View Full-Text
Keywords: Protantigius superans; Spindasis takanosis; endangered species; transcriptome; Illumina sequencing; BLAST2GO; SSRs (simple sequence repeats) Protantigius superans; Spindasis takanosis; endangered species; transcriptome; Illumina sequencing; BLAST2GO; SSRs (simple sequence repeats)
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MDPI and ACS Style

Patnaik, B.B.; Hwang, H.-J.; Kang, S.W.; Park, S.Y.; Wang, T.H.; Park, E.B.; Chung, J.M.; Song, D.K.; Kim, C.; Kim, S.; Lee, J.B.; Jeong, H.C.; Park, H.S.; Han, Y.S.; Lee, Y.S. Transcriptome Characterization for Non-Model Endangered Lycaenids, Protantigius superans and Spindasis takanosis, Using Illumina HiSeq 2500 Sequencing. Int. J. Mol. Sci. 2015, 16, 29948-29970. https://doi.org/10.3390/ijms161226213

AMA Style

Patnaik BB, Hwang H-J, Kang SW, Park SY, Wang TH, Park EB, Chung JM, Song DK, Kim C, Kim S, Lee JB, Jeong HC, Park HS, Han YS, Lee YS. Transcriptome Characterization for Non-Model Endangered Lycaenids, Protantigius superans and Spindasis takanosis, Using Illumina HiSeq 2500 Sequencing. International Journal of Molecular Sciences. 2015; 16(12):29948-29970. https://doi.org/10.3390/ijms161226213

Chicago/Turabian Style

Patnaik, Bharat Bhusan, Hee-Ju Hwang, Se Won Kang, So Young Park, Tae Hun Wang, Eun Bi Park, Jong Min Chung, Dae Kwon Song, Changmu Kim, Soonok Kim, Jae Bong Lee, Heon Cheon Jeong, Hong Seog Park, Yeon Soo Han, and Yong Seok Lee. 2015. "Transcriptome Characterization for Non-Model Endangered Lycaenids, Protantigius superans and Spindasis takanosis, Using Illumina HiSeq 2500 Sequencing" International Journal of Molecular Sciences 16, no. 12: 29948-29970. https://doi.org/10.3390/ijms161226213

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