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Int. J. Mol. Sci. 2015, 16(12), 29345-29356;

The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China
Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China
Takarazuka University of Medical and Health Care, Hanayashiki-Midorigaoka, Takarazuka-City 6660162, Japan
East China University of Science and Technology Shanghai, Meilong Road 130, Shanghai 200237, China
Authors to whom correspondence should be addressed.
Academic Editor: Ge Zhang
Received: 30 September 2015 / Revised: 5 November 2015 / Accepted: 17 November 2015 / Published: 9 December 2015
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Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety of the combined SalB and Rg1 (SalB-Rg1) in mice. The safety of SalB-Rg1 was evaluated through acute toxicity and repeated-dose toxicity. In the acute toxicity study, the up and down procedure was carried out firstly, and then, the Bliss method was applied. In the toxicity study for seven-day repeated treatment of SalB-Rg1, forty Kunming mice were randomly divided into four groups. The intravenous median lethal dose (LD50) of the SalB-Rg1 combination was 1747 mg/kg using the Bliss method. For both the acute toxicity study and the seven-day repeated toxicity study, SalB-Rg1 did not induce significant abnormality on brain, heart, kidney, liver and lung structure at any dose based on H&E stain. There were no significant changes related to the SalB-Rg1 toxicity detected on biochemical parameters for two kinds of toxicity studies. The LD50 in mice was 1747 mg/kg, which was more than one hundred times higher than the effective dose. Both studies of acute toxicity and seven-day repeated dose toxicity indicated the safety of the SalB-Rg1 combination. View Full-Text
Keywords: safety; salvianolic acid B; ginsenoside Rg1; acute toxicity; repeated toxicity safety; salvianolic acid B; ginsenoside Rg1; acute toxicity; repeated toxicity

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Zhao, Q.; Yang, M.; Deng, Y.; Yu, H.; Wang, L.; Teng, F.; Cho, K.; Ma, H.; Wu, P.; Li, X.; Wu, W.; Liu, X.; Xu, F.; Jiang, B.; Guo, D.-A. The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice. Int. J. Mol. Sci. 2015, 16, 29345-29356.

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