Next Article in Journal
DNA Double Strand Break Response and Limited Repair Capacity in Mouse Elongated Spermatids
Next Article in Special Issue
Diabetes Mellitus Induces Alzheimer’s Disease Pathology: Histopathological Evidence from Animal Models
Previous Article in Journal
Effect of Guibi-Tang, a Traditional Herbal Formula, on Retinal Neovascularization in a Mouse Model of Proliferative Retinopathy
Previous Article in Special Issue
Leptin Promotes cPLA2 Gene Expression through Activation of the MAPK/NF-κB/p300 Cascade
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 16
Issue 12
10.3390/ijms161226206
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women

by
Esther Guiu-Jurado
1,†,
Teresa Auguet
1,2,†,
Alba Berlanga
1,
Gemma Aragonès
1,
Carmen Aguilar
1,
Fàtima Sabench
3,
Sandra Armengol
1,
José Antonio Porras
2,
Andreu Martí
2,
Rosa Jorba
4,
Mercè Hernández
3,
Daniel Del Castillo
3 and
Cristóbal Richart
1,2,*
1
Grup de Recerca GEMMAIR (AGAUR)—Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d’Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain
2
Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain
3
Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d’Investigació Pere Virgili (IISPV), Avinguda Doctor Josep Laporte, 2, 43204 Reus, Spain
4
Servei de Cirurgia, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2015, 16(12), 29911-29922; https://doi.org/10.3390/ijms161226206
Submission received: 9 October 2015 / Revised: 13 November 2015 / Accepted: 4 December 2015 / Published: 16 December 2015
(This article belongs to the Special Issue Molecular Research on Obesity and Diabetes)
Browse Figures
Versions Notes

Abstract

The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPARα, PPARδ) and inflammation (IL6, TNFα), in normal weight control women (BMI < 25 kg/m2, n = 35) and moderately obese women (BMI 30–38 kg/m2, n = 55). In SAT, ACC1, FAS and PPARα mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPARα were similar in both groups. Only PPARδ gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNFα and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity.
Keywords: moderate obesity; fatty acid metabolism; adipose tissue; de novo fatty acid synthesis moderate obesity; fatty acid metabolism; adipose tissue; de novo fatty acid synthesis
Graphical Abstract

Share and Cite

MDPI and ACS Style

Guiu-Jurado, E.; Auguet, T.; Berlanga, A.; Aragonès, G.; Aguilar, C.; Sabench, F.; Armengol, S.; Porras, J.A.; Martí, A.; Jorba, R.; et al. Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women. Int. J. Mol. Sci. 2015, 16, 29911-29922. https://doi.org/10.3390/ijms161226206

AMA Style

Guiu-Jurado E, Auguet T, Berlanga A, Aragonès G, Aguilar C, Sabench F, Armengol S, Porras JA, Martí A, Jorba R, et al. Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women. International Journal of Molecular Sciences. 2015; 16(12):29911-29922. https://doi.org/10.3390/ijms161226206

Chicago/Turabian Style

Guiu-Jurado, Esther, Teresa Auguet, Alba Berlanga, Gemma Aragonès, Carmen Aguilar, Fàtima Sabench, Sandra Armengol, José Antonio Porras, Andreu Martí, Rosa Jorba, and et al. 2015. "Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women" International Journal of Molecular Sciences 16, no. 12: 29911-29922. https://doi.org/10.3390/ijms161226206

APA Style

Guiu-Jurado, E., Auguet, T., Berlanga, A., Aragonès, G., Aguilar, C., Sabench, F., Armengol, S., Porras, J. A., Martí, A., Jorba, R., Hernández, M., Del Castillo, D., & Richart, C. (2015). Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women. International Journal of Molecular Sciences, 16(12), 29911-29922. https://doi.org/10.3390/ijms161226206

Article Metrics

Back to TopTop