MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
Abstract
:1. Introduction
2. Results
2.1. MiR542-3p Expression Was Up-Regulated in Fibrotic Kidney
2.2. Identification of TGFβ1 as a Positive Regulator of MiR542-3p Expression
2.3. MiR542-3p Induce Epithelial-to-Mesenchymal Transition (EMT)
2.4. BMP7 Is a Direct Target of MiR542-3p
2.5. Overexpression of BMP7 Attenuates the MiR542-3p-Induced EMT
3. Discussion
4. Experimental Section
4.1. Obstructive Kidney Disease Model
4.2. Cell Culture and Transfection
4.3. RNA Isolation and Quantitative Real-Time PCR Analysis
Gene Name | Sense | Antisense |
---|---|---|
BMP7 | CAGCCACCAGCAACCACT | GTCCATGCCGTCCAATCA |
Ecadherin | GTCAACACCTACAACGCTGC | ACGTGCTTGGGTTGAAGACA |
Vimentin | TGACCGCTTCGCCAACTA | CGCAACTCCCTCATCTCCT |
Collagen I | AACTTTGCTTCCCAGATFTCCTATG | GCTTCCCCATCATCTCCATTCTTGC |
a-SMA | GGGGTGATGGTGGGAATG | GGGGTGATGGTGGGAATG |
Fibnectin | TGTGACCCAGACTTACGG | TGTAGGTGAACGGGAGAA |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
18sRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
4.4. Western Bolt Analysis
4.5. Dual Luciferase Reporter Assay
4.6. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Liu, Z.; Zhou, Y.; Yuan, Y.; Nie, F.; Peng, R.; Li, Q.; Lyu, Z.; Mao, Z.; Huang, L.; Zhou, L.; et al. MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e. Int. J. Mol. Sci. 2015, 16, 27945-27955. https://doi.org/10.3390/ijms161126075
Liu Z, Zhou Y, Yuan Y, Nie F, Peng R, Li Q, Lyu Z, Mao Z, Huang L, Zhou L, et al. MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e. International Journal of Molecular Sciences. 2015; 16(11):27945-27955. https://doi.org/10.3390/ijms161126075
Chicago/Turabian StyleLiu, Zhicheng, Yuru Zhou, Yue Yuan, Fang Nie, Rui Peng, Qianyin Li, Zhongshi Lyu, Zhaomin Mao, Liyuan Huang, Li Zhou, and et al. 2015. "MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e" International Journal of Molecular Sciences 16, no. 11: 27945-27955. https://doi.org/10.3390/ijms161126075