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Article

Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage

by
Alessandro R. Zorzi
1,2,*,
Eliane M. I. Amstalden
3,
Ana Maria G. Plepis
4,
Virginia C. A. Martins
4,
Mario Ferretti
2,
Eliane Antonioli
2,
Adriana S. S. Duarte
5,
Angela C. M. Luzo
5 and
João B. Miranda
1
1
Department of Orthopedic Surgery, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil
2
Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil
3
Department of Pathology, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil
4
Instituto de Química de São Carlos, Universidade de São Paulo (USP), São Carlos/SP 13566-590, Brazil
5
Hematology Hemotherapy Centre, Universidade de Campinas (UNICAMP), Campinas/SP 13083-878, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2015, 16(11), 26813-26831; https://doi.org/10.3390/ijms161125989
Submission received: 10 September 2015 / Revised: 12 October 2015 / Accepted: 15 October 2015 / Published: 9 November 2015
(This article belongs to the Special Issue Molecular and Cellular Basis of Regeneration and Tissue Repair)
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Abstract

Cell therapy is a promising approach to improve cartilage healing. Adipose tissue is an abundant and readily accessible cell source. Previous studies have demonstrated good cartilage repair results with adipose tissue mesenchymal stem cells in small animal experiments. This study aimed to examine these cells in a large animal model. Thirty knees of adult sheep were randomly allocated to three treatment groups: CELLS (scaffold seeded with human adipose tissue mesenchymal stem cells), SCAFFOLD (scaffold without cells), or EMPTY (untreated lesions). A partial thickness defect was created in the medial femoral condyle. After six months, the knees were examined according to an adaptation of the International Cartilage Repair Society (ICRS 1) score, in addition to a new Partial Thickness Model scale and the ICRS macroscopic score. All of the animals completed the follow-up period. The CELLS group presented with the highest ICRS 1 score (8.3 ± 3.1), followed by the SCAFFOLD group (5.6 ± 2.2) and the EMPTY group (5.2 ± 2.4) (p = 0.033). Other scores were not significantly different. These results suggest that human adipose tissue mesenchymal stem cells promoted satisfactory cartilage repair in the ovine model.
Keywords: tissue engineering; regenerative medicine; adult stem cell; stem cell therapy; biomaterials; cartilage tissue engineering; regenerative medicine; adult stem cell; stem cell therapy; biomaterials; cartilage

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MDPI and ACS Style

Zorzi, A.R.; Amstalden, E.M.I.; Plepis, A.M.G.; Martins, V.C.A.; Ferretti, M.; Antonioli, E.; Duarte, A.S.S.; Luzo, A.C.M.; Miranda, J.B. Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage. Int. J. Mol. Sci. 2015, 16, 26813-26831. https://doi.org/10.3390/ijms161125989

AMA Style

Zorzi AR, Amstalden EMI, Plepis AMG, Martins VCA, Ferretti M, Antonioli E, Duarte ASS, Luzo ACM, Miranda JB. Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage. International Journal of Molecular Sciences. 2015; 16(11):26813-26831. https://doi.org/10.3390/ijms161125989

Chicago/Turabian Style

Zorzi, Alessandro R., Eliane M. I. Amstalden, Ana Maria G. Plepis, Virginia C. A. Martins, Mario Ferretti, Eliane Antonioli, Adriana S. S. Duarte, Angela C. M. Luzo, and João B. Miranda. 2015. "Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage" International Journal of Molecular Sciences 16, no. 11: 26813-26831. https://doi.org/10.3390/ijms161125989

APA Style

Zorzi, A. R., Amstalden, E. M. I., Plepis, A. M. G., Martins, V. C. A., Ferretti, M., Antonioli, E., Duarte, A. S. S., Luzo, A. C. M., & Miranda, J. B. (2015). Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage. International Journal of Molecular Sciences, 16(11), 26813-26831. https://doi.org/10.3390/ijms161125989

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