Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Bisphenol A (BPA) Alters Global Transcription in HT29 Human Colon Adenocarcinona Cell Line and Human Umbilical Vein Endothelial Cells (HUVEC)
2.2. Continuous BPA Exposure Reduces Viability in Aging HUVEC
2.3. Continuous BPA Exposure Induces Differential Gene Expression in Aging HUVEC
3. Experimental Section
3.1. Cell Cultures and Reagents
3.2. BPA Treatments and Controls
3.3. cDNA Isolation and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
Sequence | Accession No. | Forward Primer (5'→3') | Reverse Primer (5'→3') |
---|---|---|---|
L1-5' [24] | M80340.1 | GGCCAGTGTGTGTGCGCACCG | CCAGGTGTGGGATATAGTCTCGTGG |
L1-3' [24] | M80340.1 | CAGGAAGGGGAATATCACACTC | TGCGCTGCACCCACTAACTC |
SPARC | NM_003118 | CTGTGGGAGCTAATCCTG | GGGTGCTGGTCCAGCTGG |
FN1 | NM_002026 | TGTGGTTGCCTTGCACGAT | GCTTGTGGGTGTGACCTGAGT |
p21 | NM_000389 | CTGGAGACTCTCAGGGTCGAA | CCAGGACTGCAGGCTTCCT |
FOS | NM_005252 | AGGAGAATCCGAAGGGAAAG | CAAGGGAAGCCACAGACATC |
bcl-xL | NM_001191 | TTACCTGAATGACCACCTA | ATTTCCGACTGAAGAGTGA |
NCL | NM_005381 | CCTTCTGAGGACATTCCAAGACA | ACGGTATTGCCCTTGAAATGTT |
18SrRNA | NR_003286 | CATTCGAACGTCTGCCCTAT | CCTCCAATGGATCCTCGTTA |
GAPDH | NM_002046 | GAGTCAACGGATTTGGTCGTA | GCAGAGATGATGACCCTTTTG |
β-actin | NM_001101 | GGTCATCTTCTCGCGGTTGGCCTTGGGGT | CCCCAGGCACCAGGGCGTGAT |
3.4. Protein Extraction and Western Blotting Analysis
3.5. Cell Viability Assay
3.6. Immunofluorescence
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ribeiro-Varandas, E.; Pereira, H.S.; Monteiro, S.; Neves, E.; Brito, L.; Ferreira, R.B.; Viegas, W.; Delgado, M. Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells. Int. J. Mol. Sci. 2014, 15, 15791-15805. https://doi.org/10.3390/ijms150915791
Ribeiro-Varandas E, Pereira HS, Monteiro S, Neves E, Brito L, Ferreira RB, Viegas W, Delgado M. Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells. International Journal of Molecular Sciences. 2014; 15(9):15791-15805. https://doi.org/10.3390/ijms150915791
Chicago/Turabian StyleRibeiro-Varandas, Edna, H. Sofia Pereira, Sara Monteiro, Elsa Neves, Luísa Brito, Ricardo Boavida Ferreira, Wanda Viegas, and Margarida Delgado. 2014. "Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells" International Journal of Molecular Sciences 15, no. 9: 15791-15805. https://doi.org/10.3390/ijms150915791