Next Article in Journal
Geminal Brønsted Acid Ionic Liquids as Catalysts for the Mannich Reaction in Water
Previous Article in Journal
Evolving Concepts in the Pathogenesis of NASH: Beyond Steatosis and Inflammation
Article

Sub-Inhibitory Concentrations of Trans-Cinnamaldehyde Attenuate Virulence in Cronobacter sakazakii in Vitro

1
Department of Animal Science, University of Connecticut, 3636 Horse Barn Hill Road Ext., Unit 4040, Storrs, CT 06269, USA
2
Division of Pediatric Infectious Diseases, School of Medicine, Johns Hopkins University, Baltimore, MD 21218, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(5), 8639-8655; https://doi.org/10.3390/ijms15058639
Received: 5 April 2014 / Revised: 28 April 2014 / Accepted: 5 May 2014 / Published: 15 May 2014
(This article belongs to the Section Biochemistry)
Cronobacter sakazakii is a foodborne pathogen, which causes a life-threatening form of meningitis, necrotizing colitis and meningoencephalitis in neonates and children. Epidemiological studies implicate dried infant formula as the principal source of C. sakazakii. In this study, we investigated the efficacy of sub-inhibitory concentrations (SIC) of trans-cinnamaldehyde (TC), an ingredient in cinnamon, for reducing C. sakazakii virulence in vitro using cell culture, microscopy and gene expression assays. TC significantly (p ≤ 0.05) suppressed C. sakazakii adhesion to and invasion of human and rat intestinal epithelial cells, and human brain microvascular endothelial cells. In addition, TC inhibited C. sakazakii survival and replication in human macrophages. We also observed that TC reduced the ability of C. sakazakii to cause cell death in rat intestinal cells, by inhibiting nitric oxide production. Results from gene expression studies revealed that TC significantly downregulated the virulence genes critical for motility, host tissue adhesion and invasion, macrophage survival, and LPS (Lipopolysaccharide) synthesis in C. sakazakii. The efficacy of TC in attenuating these major virulence factors in C. sakazakii underscores its potential use in the prevention and/or control of infection caused by this pathogen. View Full-Text
Keywords: C. sakazakii; trans-cinnamaldehyde; sub-inhibitory concentration; virulence; attenuation C. sakazakii; trans-cinnamaldehyde; sub-inhibitory concentration; virulence; attenuation
Show Figures

MDPI and ACS Style

Amalaradjou, M.A.R.; Kim, K.S.; Venkitanarayanan, K. Sub-Inhibitory Concentrations of Trans-Cinnamaldehyde Attenuate Virulence in Cronobacter sakazakii in Vitro. Int. J. Mol. Sci. 2014, 15, 8639-8655. https://doi.org/10.3390/ijms15058639

AMA Style

Amalaradjou MAR, Kim KS, Venkitanarayanan K. Sub-Inhibitory Concentrations of Trans-Cinnamaldehyde Attenuate Virulence in Cronobacter sakazakii in Vitro. International Journal of Molecular Sciences. 2014; 15(5):8639-8655. https://doi.org/10.3390/ijms15058639

Chicago/Turabian Style

Amalaradjou, Mary Anne R., Kwang S. Kim, and Kumar Venkitanarayanan. 2014. "Sub-Inhibitory Concentrations of Trans-Cinnamaldehyde Attenuate Virulence in Cronobacter sakazakii in Vitro" International Journal of Molecular Sciences 15, no. 5: 8639-8655. https://doi.org/10.3390/ijms15058639

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop