The Canonical Notch Signaling Was Involved in the Regulation of Intestinal Epithelial Cells Apoptosis after Intestinal Ischemia/Reperfusion Injury
Abstract
:1. Introduction
2. Results
2.1. Intestinal I/R Injury Increased Apoptosis of Intestinal Epithelial Cells
2.2. The Notch Signaling Was Activated in a Mouse Model of Intestinal I/R
2.3. Effects of DAPT on the Apoptosis of IEC-6 Cells
2.4 Effects of Silencing RNA for Hes5 on the Apoptosis of IEC-6 Cells
3. Discussion
4. Experimental Section
4.1. Animals
4.2. Cell Culture
4.3. Real-Time PCR Analysis
4.4. Western Blot Analysis
4.5. Silencing Hes5 Using siRNA and in Vitro Transfection
4.6. Detection of Epithelial Apoptosis
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Chen, G.; Zhang, Z.; Cheng, Y.; Xiao, W.; Qiu, Y.; Yu, M.; Sun, L.; Wang, W.; Du, G.; Gu, Y.; et al. The Canonical Notch Signaling Was Involved in the Regulation of Intestinal Epithelial Cells Apoptosis after Intestinal Ischemia/Reperfusion Injury. Int. J. Mol. Sci. 2014, 15, 7883-7896. https://doi.org/10.3390/ijms15057883
Chen G, Zhang Z, Cheng Y, Xiao W, Qiu Y, Yu M, Sun L, Wang W, Du G, Gu Y, et al. The Canonical Notch Signaling Was Involved in the Regulation of Intestinal Epithelial Cells Apoptosis after Intestinal Ischemia/Reperfusion Injury. International Journal of Molecular Sciences. 2014; 15(5):7883-7896. https://doi.org/10.3390/ijms15057883
Chicago/Turabian StyleChen, Guoqing, Zhicao Zhang, Yingdong Cheng, Weidong Xiao, Yuan Qiu, Min Yu, Lihua Sun, Wensheng Wang, Guangsheng Du, Yingchao Gu, and et al. 2014. "The Canonical Notch Signaling Was Involved in the Regulation of Intestinal Epithelial Cells Apoptosis after Intestinal Ischemia/Reperfusion Injury" International Journal of Molecular Sciences 15, no. 5: 7883-7896. https://doi.org/10.3390/ijms15057883