[FeFe]-Hydrogenase Abundance and Diversity along a Vertical Redox Gradient in Great Salt Lake, USA
Abstract
:1. Introduction
2. Results
2.1. Water Column Chemistry
Depth (m) | Latitude | Longitude | Salinity (ppt) | Dissolved | PAR (µmol·photons·m–2·s−1) | Temperature (°C) | pH |
---|---|---|---|---|---|---|---|
O2 (mg/L) | |||||||
0.0 | 41.1674600 | −112.6696117 | 149 | 5.46 | 2079 | 24.17 | 7.21 |
1.0 | 41.1674600 | −112.6696117 | 151 | 5.38 | 1219 | 24.16 | 7.46 |
4.0 | 41.1674717 | −112.6696117 | 143 | 5.13 | 175 | 23.77 | 7.83 |
6.0 | 41.1674717 | −112.6696417 | 151 | 2.32 | 75 | 23.37 | 8.07 |
6.5 | 41.1674833 | −112.6696200 | 203 | 1.68 | 44 | 20.20 b | 7.10 b |
8.0 | 41.1674817 | −112.6696083 | 247 | 0.98 | 0 | 16.59 | 5.95 |
2.2. Abundance, Composition, and Diversity of hydA in GSL Water Column
Depth (m) | Templates/ng DNA | SD | na | PD b |
---|---|---|---|---|
0.0 | BD | − | − | − |
1.0 | BD | − | − | − |
4.0 | BD | − | − | − |
6.0 | 1.6 × 105 | 1.0 × 104 | 58 | 12.5 |
6.5 | 1.9 × 105 | 3.0 × 104 | 55 | 12.5 |
8.0 | 5.9 × 105 | 3.1 × 104 | 53 | 13.9 |
Benthic c | 9.2 × 105 | 1.9 × 104 | 59 | 16.0 |
2.3. Variation in H-Cluster Binding Motifs and Putative Gas Channels
2.4. Inferred Structural Variation of GSL HydA
3. Discussion
4. Experimental Section
4.1. Site Description and Sample Collection
4.2. Physical and Chemical Analysis
4.3. DNA Extraction, Amplification, Cloning, and Sequencing of hydA
4.4. Primary Sequence Analysis
4.5. Prediction of Environmental HydA Accessary Cluster Composition
4.6. hydA Quantitative PCR (qPCR)
4.7. HydA Phylogenetic Diversity
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Boyd, E.S.; Hamilton, T.L.; Swanson, K.D.; Howells, A.E.; Baxter, B.K.; Meuser, J.E.; Posewitz, M.C.; Peters, J.W. [FeFe]-Hydrogenase Abundance and Diversity along a Vertical Redox Gradient in Great Salt Lake, USA. Int. J. Mol. Sci. 2014, 15, 21947-21966. https://doi.org/10.3390/ijms151221947
Boyd ES, Hamilton TL, Swanson KD, Howells AE, Baxter BK, Meuser JE, Posewitz MC, Peters JW. [FeFe]-Hydrogenase Abundance and Diversity along a Vertical Redox Gradient in Great Salt Lake, USA. International Journal of Molecular Sciences. 2014; 15(12):21947-21966. https://doi.org/10.3390/ijms151221947
Chicago/Turabian StyleBoyd, Eric S., Trinity L. Hamilton, Kevin D. Swanson, Alta E. Howells, Bonnie K. Baxter, Jonathan E. Meuser, Matthew C. Posewitz, and John W. Peters. 2014. "[FeFe]-Hydrogenase Abundance and Diversity along a Vertical Redox Gradient in Great Salt Lake, USA" International Journal of Molecular Sciences 15, no. 12: 21947-21966. https://doi.org/10.3390/ijms151221947