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Int. J. Mol. Sci. 2014, 15(12), 21935-21946;

Role of Aeromonas hydrophila Flagella Glycosylation in Adhesion to Hep-2 Cells, Biofilm Formation and Immune Stimulation

Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain
Author to whom correspondence should be addressed.
Received: 4 September 2014 / Revised: 13 November 2014 / Accepted: 21 November 2014 / Published: 28 November 2014
(This article belongs to the Special Issue Bioactive Carbohydrates and Peptides)
PDF [1082 KB, uploaded 28 November 2014]


Polar flagellin proteins from Aeromonas hydrophila strain AH-3 (serotype O34) were found to be O-glycosylated with a heterogeneous heptasaccharide glycan. Two mutants with altered (light and strong) polar flagella glycosylation still able to produce flagella were previously obtained, as well as mutants lacking the O34-antigen lipopolysaccharide (LPS) but with unaltered polar flagella glycosylation. We compared these mutants, altogether with the wild type strain, in different studies to conclude that polar flagella glycosylation is extremely important for A. hydrophila adhesion to Hep-2 cells and biofilm formation. Furthermore, the polar flagella glycosylation is an important factor for the immune stimulation of IL-8 production via toll receptor 5 (TLR5). View Full-Text
Keywords: O-flagellin glycosylation; adhesion; biofilm; immune stimulation O-flagellin glycosylation; adhesion; biofilm; immune stimulation

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Merino, S.; Wilhelms, M.; Tomás, J.M. Role of Aeromonas hydrophila Flagella Glycosylation in Adhesion to Hep-2 Cells, Biofilm Formation and Immune Stimulation. Int. J. Mol. Sci. 2014, 15, 21935-21946.

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