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Open AccessArticle

Quorum Quenching in Culturable Phyllosphere Bacteria from Tobacco

by 1,†, 2,†, 1 and 1,*
1
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2
Insitute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2013, 14(7), 14607-14619; https://doi.org/10.3390/ijms140714607
Received: 30 May 2013 / Revised: 25 June 2013 / Accepted: 26 June 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Quorum Sensing Research in Microbial Systems)
Many Gram-negative plant pathogenic bacteria employ a N-acylhomoserine lactone (AHL)-based quorum sensing (QS) system to regulate their virulence traits. A sustainable biocontrol strategy has been developed using quorum quenching (QQ) bacteria to interfere with QS and protect plants from pathogens. Here, the prevalence and the diversity of QQ strains inhabiting tobacco leaf surfaces were explored. A total of 1177 leaf-associated isolates were screened for their ability to disrupt AHL-mediated QS, using the biosensor Chromobacterium violaceum CV026. One hundred and sixty-eight strains (14%) are capable of interfering with AHL activity. Among these, 106 strains (63%) of the culturable quenchers can enzymatically degrade AHL molecules, while the remaining strains might use other QS inhibitors to interrupt the chemical communication. Moreover, almost 79% of the QQ strains capable of inactivating AHLs enzymatically have lactonase activity. Further phylogenetic analysis based on 16S rDNA revealed that the leaf-associated QQ bacteria can be classified as Bacillus sp., Acinetobacter sp., Lysinibacillus sp., Serratia sp., Pseudomonas sp., and Myroides sp. The naturally occurring diversity of bacterial quenchers might provide opportunities to use them as effective biocontrol reagents for suppressing plant pathogen in situ. View Full-Text
Keywords: quorum quenching; bacteria; phyllosphere; biosassay; N-acyl-homoserine lactone quorum quenching; bacteria; phyllosphere; biosassay; N-acyl-homoserine lactone
MDPI and ACS Style

Ma, A.; Lv, D.; Zhuang, X.; Zhuang, G. Quorum Quenching in Culturable Phyllosphere Bacteria from Tobacco. Int. J. Mol. Sci. 2013, 14, 14607-14619. https://doi.org/10.3390/ijms140714607

AMA Style

Ma A, Lv D, Zhuang X, Zhuang G. Quorum Quenching in Culturable Phyllosphere Bacteria from Tobacco. International Journal of Molecular Sciences. 2013; 14(7):14607-14619. https://doi.org/10.3390/ijms140714607

Chicago/Turabian Style

Ma, Anzhou; Lv, Di; Zhuang, Xuliang; Zhuang, Guoqiang. 2013. "Quorum Quenching in Culturable Phyllosphere Bacteria from Tobacco" Int. J. Mol. Sci. 14, no. 7: 14607-14619. https://doi.org/10.3390/ijms140714607

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