A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification
Abstract
:1. Introduction
2. Results and Discussion
2.1. LoTemp PCR Extension of Primer with Mismatched Base at the 3′-Terminus
2.2. High Amplicon Yield by LoTemp PCR
2.3. High Processivity of LoTemp PCR
3. Experimental Section
3.1. Preparation of the 449 bp HPV-52 L1 Gene MY09/MY11 Primary PCR Amplicon
3.2. LoTemp Heminested PCR Amplicon as Template for DNA Sequencing
3.3. Direct DNA Sequencing
4. Conclusions
Acknowledgments
Conflict of Interest
References
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PCR runs | 1 | 2 | 3 | 4 | 5 |
---|---|---|---|---|---|
DNA yields by T | 0.7 | 1.8 | 1.8 | 2.2 | 2.6 |
DNA yields by L | 1.7 | 4.1 | 4.6 | 5.1 | 5.1 |
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Hong, G.; Lee, S.H.; Ge, S.; Zhou, S. A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification. Int. J. Mol. Sci. 2013, 14, 12853-12862. https://doi.org/10.3390/ijms140612853
Hong G, Lee SH, Ge S, Zhou S. A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification. International Journal of Molecular Sciences. 2013; 14(6):12853-12862. https://doi.org/10.3390/ijms140612853
Chicago/Turabian StyleHong, Guofan, Sin Hang Lee, Shichao Ge, and Shaoxia Zhou. 2013. "A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification" International Journal of Molecular Sciences 14, no. 6: 12853-12862. https://doi.org/10.3390/ijms140612853