Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides
Abstract
:1. Introduction
2. Results
2.1. Optimization of the CtCVNH Gene
2.2. Construction of the Expression Strain
2.3. Protein Expression and Solubility Analysis
2.4. Protein Purification and Western Blot Analysis
2.5. PMF Analysis and Molecular Weight Determination
3. Discussion
4. Experimental Section
4.1. Experimental Materials
4.2. Sequence Optimization and Construct Design
4.3. Construction of the Expression Strain
4.4. Expression of Recombinant Protein and Solubility Analysis
4.5. Protein Purification
4.6. Western Blot Analysis
4.7. Peptide Mass Fingerprinting Analysis and Molecular Weight Determination
5. Conclusions
Acknowledgments
Conflict of Interest
References
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Sun, J.; Su, Y.; Wang, T. Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides. Int. J. Mol. Sci. 2013, 14, 7506-7514. https://doi.org/10.3390/ijms14047506
Sun J, Su Y, Wang T. Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides. International Journal of Molecular Sciences. 2013; 14(4):7506-7514. https://doi.org/10.3390/ijms14047506
Chicago/Turabian StyleSun, Junbo, Yingjuan Su, and Ting Wang. 2013. "Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides" International Journal of Molecular Sciences 14, no. 4: 7506-7514. https://doi.org/10.3390/ijms14047506