N,N’-alkylated Imidazolium-Derivatives Act as Quorum-Sensing Inhibitors Targeting the Pectobacterium atrosepticum-Induced Symptoms on Potato Tubers
Abstract
:1. Introduction
2. Results and Discussion
2.1. Construction of the Pectobacterium AHL-Biosensor
2.2. QSIs Identification
2.3. Biological Effects of the Identified QSIs on Pectobacterium Cells
2.4. QSIs Could Moderate the P. atrosepticum-Induced Symptoms in Potato Tubers
3. Experimental Section
3.1. Bacterial Strains and Growth Conditions
3.2. Chemical Library
3.3. Screening for QSIs
3.4. Measurement of AC50, GIAC50, MIC and MBC Values of the QSIs
3.5. Virulence Assays on Potato Tubers
4. Conclusions
Pectobacterium cells | ||||
---|---|---|---|---|
Name | AC50a | GIAC50b | MICa | MBCa |
4-NPO | >100 (12%) c | 1.00 | 50 | >100 |
29-L-A06 | 18 | 0.94 | 25 | 25 |
29-L-A11 | 14 | 0.93 | 10 | 25 |
29-L-B02 | 16 | 0.99 | <2.5 | 2.5 |
29-L-C03 | 20 | 0.93 | 25 | 25 |
Acknowledgments
Conflicts of Interest
Abbreviations
3-OC8-HSL | 3-oxo-octanoyl-l-homoserine lactone |
4-NPO | 4-nitropyridine-N-oxide |
AC50 | half maximal activity concentration |
AHL | N-acyl homoserine lactone |
DMSO | dimethylsulfoxide |
GIAC50 | growth index at the AC50 concentration |
MBC | minimal bactericidal concentration |
MIC | minimal inhibitory concentration |
OD600 | optical density at a wavelength of 600 nm |
Pa6276 | P. atrosepticum CFBP6276 |
QS | quorum-sensing |
QSI | quorum sensing-inhibitor |
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Des Essarts, Y.R.; Sabbah, M.; Comte, A.; Soulère, L.; Queneau, Y.; Dessaux, Y.; Hélias, V.; Faure, D. N,N’-alkylated Imidazolium-Derivatives Act as Quorum-Sensing Inhibitors Targeting the Pectobacterium atrosepticum-Induced Symptoms on Potato Tubers. Int. J. Mol. Sci. 2013, 14, 19976-19986. https://doi.org/10.3390/ijms141019976
Des Essarts YR, Sabbah M, Comte A, Soulère L, Queneau Y, Dessaux Y, Hélias V, Faure D. N,N’-alkylated Imidazolium-Derivatives Act as Quorum-Sensing Inhibitors Targeting the Pectobacterium atrosepticum-Induced Symptoms on Potato Tubers. International Journal of Molecular Sciences. 2013; 14(10):19976-19986. https://doi.org/10.3390/ijms141019976
Chicago/Turabian StyleDes Essarts, Yannick Raoul, Mohamad Sabbah, Arnaud Comte, Laurent Soulère, Yves Queneau, Yves Dessaux, Valérie Hélias, and Denis Faure. 2013. "N,N’-alkylated Imidazolium-Derivatives Act as Quorum-Sensing Inhibitors Targeting the Pectobacterium atrosepticum-Induced Symptoms on Potato Tubers" International Journal of Molecular Sciences 14, no. 10: 19976-19986. https://doi.org/10.3390/ijms141019976