Next Article in Journal
Synthesis and Antimicrobial Activity of Some Novel Cross-Linked Chitosan Hydrogels
Previous Article in Journal
Commercial Bee Pollen with Different Geographical Origins: A Comprehensive Approach
Open AccessShort Note

Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome

1
Hubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China
2
State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(9), 11188-11193; https://doi.org/10.3390/ijms130911188
Received: 6 August 2012 / Revised: 3 September 2012 / Accepted: 3 September 2012 / Published: 7 September 2012
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (HE) and observed heterozygosity (HO) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species. View Full-Text
Keywords: microsatellite markers; Phaseolus vulgaris L.; cross-species transferability; genetic diversity microsatellite markers; Phaseolus vulgaris L.; cross-species transferability; genetic diversity
MDPI and ACS Style

Wang, A.; Ding, Y.; Hu, Z.; Lin, C.; Wang, S.; Wang, B.; Zhang, H.; Zhou, G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. Int. J. Mol. Sci. 2012, 13, 11188-11193.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop