Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
4. Conclusions
Acknowledgements
Reference
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Locus | Primer Sequence (5′–3′) | Repeat Motif | Size Range (bp) | Ho | He | Na | D | PIC | Ta (°C) | GenBank |
---|---|---|---|---|---|---|---|---|---|---|
C9 | F:ACAGAGACGAGTGCGTGAGAGTTAG | (AT)15 | 445–470 | 0.469 | 0.428 | 4 | * | 0.471 | 57 | JQ739888 |
R:AAAGACAGTTCTAGGAAGAACCGTC | ||||||||||
C33 | F: CTCTTTCTGCTTCCTTTCTACGC | (AG)15 | 536–565 | 0.531 | 0.814 | 7 | * | 0.721 | 59 | JQ739889 |
R:TTCTTCACAGTCAAGGGAGTAGAAG | ||||||||||
C42 | F:TGTCATAAACCTGCTGGTGAATAAC | (AG)28 | 287–342 | 0.026 | 0.473 | 3 | NS | 0.444 | 60 | JQ739900 |
R:CCTATTTCAGAATCACAGCTATGAC | ||||||||||
C45a | F: CATGAATGATTACGATTCGAGC | (AG)20 | 106–199 | 0.162 | 0.151 | 2 | NS | 0.281 | 60 | JQ739901 |
R:TGAGTGATTACTAGTGGAACCCA | ||||||||||
C76 | F: AGGCGAAGCAAGAGGTTTTATCCAC | (CT)20 | 194–233 | 0.027 | 0.24 | 3 | * | 0.454 | 59 | JQ739890 |
R:GAAGAGGCAGAAAGAACTTACAGCG | ||||||||||
C106a | F:TTGCAGGTAGCAGGTTGT | (TCTA)3TCTG | 383–431 | 0.029 | 0.585 | 5 | * | 0.656 | 57 | JQ739891 |
R:CAGACAGATAGATAGAGACGG | (TCTA)3TCAA(TCTA)5 | |||||||||
C115a | F: CGTAGTCTCTTTCGTCCTTTTCTGC | (CT)17 | 212–245 | 0.114 | 0.533 | 3 | * | 0.575 | 57 | JQ739892 |
R: TTACATGCCCTTTCCCTCGTTTG | ||||||||||
C117 | F: GTACCTCCTTTTGAGTTTGTAAGG | (CT)12 | 150–173 | 0.028 | 0.406 | 3 | * | 0.55 | 55 | JQ739893 |
R: GTTCGTAAGCCTACTTTCTCA | ||||||||||
C119 | F: CCACCATTGCTCTCAGTGTTA | (CT)21 | 251–292 | 0.139 | 0.45 | 3 | * | 0.458 | 57 | JQ739894 |
R: TAGATGTGTGTTTGTGTTCCG | ||||||||||
C130a | F: CCATTTCAAAGCAAACCCCTT | (CT)3CG(CT)2TG (CT)19 | 298–349 | 0.028 | 0.597 | 3 | * | 0.590 | 60 | JQ739896 |
R: TGACCCGCGATTATTTACCAC | ||||||||||
C132a | F: CAGTGGTTATTCTGGGGATT | (CT)13 | 477–502 | 0.118 | 0.674 | 5 | * | 0.687 | 58 | JQ739897 |
R: GGTTGTTTATGGCAGTAGCA | ||||||||||
C136a | F:GTAAAAGTCTCCTTCTACTTTCCCC | (CT)22 | 272–315 | 0.057 | 0.306 | 4 | * | 0.503 | 60 | JQ739898 |
R:CTCTCAAGCATGTTTGGATTGTAGC | ||||||||||
G10a | F: TCTTCTGTCCATCCCTCCATACT | (AG)27 | 220–273 | 0.088 | 0.327 | 3 | * | 0.444 | 60 | JQ739899 |
R: GATTGGTGGAAATCGACTTGTCT |
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Wang, A.; Ding, Y.; Hu, Z.; Lin, C.; Wang, S.; Wang, B.; Zhang, H.; Zhou, G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. Int. J. Mol. Sci. 2012, 13, 11188-11193. https://doi.org/10.3390/ijms130911188
Wang A, Ding Y, Hu Z, Lin C, Wang S, Wang B, Zhang H, Zhou G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. International Journal of Molecular Sciences. 2012; 13(9):11188-11193. https://doi.org/10.3390/ijms130911188
Chicago/Turabian StyleWang, Aihua, Yi Ding, Zhenhua Hu, Chufa Lin, Shuzhen Wang, Bingcai Wang, Hongyuan Zhang, and Guolin Zhou. 2012. "Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome" International Journal of Molecular Sciences 13, no. 9: 11188-11193. https://doi.org/10.3390/ijms130911188