Arsenic Trioxide Inhibits Cell Growth and Induces Apoptosis through Inactivation of Notch Signaling Pathway in Breast Cancer
Abstract
:1. Introduction
2. Results
2.1. As2O3 Inhibited Breast Cancer Cell Growth
2.2. As2O3 Induced Apoptosis in Breast Cancer Cell Lines
2.3. As2O3 Suppressed Breast Cancer Cell Invasion
2.4. As2O3 Inhibited the Notch-1 Expression in Breast Cancer Cells
2.5. As2O3 Inhibited the Expression of Notch-1 Downstream Genes
2.6. Down-Regulation of Notch-1 Expression by SiRNA and the Effect of As2O3 Treatment
2.7. Over-Expression of Notch-1 by cDNA Transfection Reduced As2O3-Induced Cell Growth Inhibition and Apoptosis
3. Discussion
4. Experiment Section
4.1. Cell Lines and Experimental Reagents
4.2. Cell Growth Inhibition Studies by MTT Assay
4.3. Histone-DNA Enzyme-Linked Immunosorbent Assay (ELISA) for Detecting Apoptosis
4.4. Annexin V-FITC Method for Apoptosis Analysis
4.5. Cell Invasion Assay
4.6. Reverse Transcription-PCR (RT-PCR) Analysis for Gene Expression Studies
4.7. Western Blot Analysis
4.8. Plasmids and Transfections
4.9. Densitometric and Statistical Analysis
5. Conclusions
Acknowledgement
- Conflict of InterestThe authors declare that they have no conflict of interest.
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Xia, J.; Li, Y.; Yang, Q.; Mei, C.; Chen, Z.; Bao, B.; Ahmad, A.; Miele, L.; Sarkar, F.H.; Wang, Z. Arsenic Trioxide Inhibits Cell Growth and Induces Apoptosis through Inactivation of Notch Signaling Pathway in Breast Cancer. Int. J. Mol. Sci. 2012, 13, 9627-9641. https://doi.org/10.3390/ijms13089627
Xia J, Li Y, Yang Q, Mei C, Chen Z, Bao B, Ahmad A, Miele L, Sarkar FH, Wang Z. Arsenic Trioxide Inhibits Cell Growth and Induces Apoptosis through Inactivation of Notch Signaling Pathway in Breast Cancer. International Journal of Molecular Sciences. 2012; 13(8):9627-9641. https://doi.org/10.3390/ijms13089627
Chicago/Turabian StyleXia, Jun, Youjian Li, Qingling Yang, Chuanzhong Mei, Zhiwen Chen, Bin Bao, Aamir Ahmad, Lucio Miele, Fazlul H Sarkar, and Zhiwei Wang. 2012. "Arsenic Trioxide Inhibits Cell Growth and Induces Apoptosis through Inactivation of Notch Signaling Pathway in Breast Cancer" International Journal of Molecular Sciences 13, no. 8: 9627-9641. https://doi.org/10.3390/ijms13089627