Quantitative Expression of C-Type Lectin Receptors in Humans and Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. C-Type Lectin Receptor mRNA Expression in Adult Human Tissues
2.2. C-Type Lectin Receptor mRNA Expression in Adult Murine Tissues
2.3. C-Type Lectin Receptor mRNA Expression in Ischemia-Reperfusion Injury (IRI)
2.4. C-Type Lectin Receptor mRNA Expression in Progressive Renal Fibrosis
2.5. C-Type Lectin Receptor mRNA Expression in Systemic Autoimmunity and Immune Complex Glomerulonephritis
2.6. Role of C-Type Lectin Receptors in Tissue Homeostasis
3. Experimental Section
3.1. Animal Studies
3.2. Human Solid Organ cDNA
3.3. Quantitative Real-Time RT-PCR
3.4. Morphological Evaluation
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
ijms-13-10113-s001.pdfAcknowledgments
References
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GAPDH | Actin | α-tubulin | Phospholipase A2 | β-mikroglobulin | |
---|---|---|---|---|---|
N | 11 | 11 | 11 | 11 | 11 |
GM | 20.35 | 17.97 | 18.98 | 20.55 | 16.49 |
AM | 20.36 | 18.01 | 19.01 | 20.60 | 16.58 |
Minimum | 19.12 | 17.35 | 17.96 | 18.39 | 13.71 |
Maximum | 21.59 | 20.34 | 20.40 | 23.88 | 21.13 |
SD | 0.75 | 1.17 | 1.12 | 1.49 | 1.84 |
Variance | 0.56 | 1.36 | 1.26 | 2.21 | 3.38 |
CV | 0.11 | 0.24 | 0.24 | 0.45 | 0.56 |
GAPDH | Actin | α-tubulin | Phospholipase A2 | β-mikroglobulin | 18 s | |
---|---|---|---|---|---|---|
N | 11 | 11 | 11 | 11 | 11 | 11 |
GM | 20.33 | 18.46 | 20.27 | 20.83 | 18.24 | 8.32 |
AM | 20.34 | 18.49 | 20.29 | 20.88 | 18.37 | 8.40 |
Minimum | 19.31 | 16.85 | 18.98 | 18.75 | 15.28 | 7.33 |
Maximum | 21.38 | 20.01 | 21.28 | 23.84 | 21.07 | 11.22 |
SD | 0.61 | 1.03 | 0.75 | 1.58 | 2.25 | 1.24 |
Variance | 0.38 | 1.07 | 0.56 | 2.50 | 5.08 | 1.53 |
CV | 0.08 | 0.20 | 0.11 | 0.52 | 0.93 | 0.13 |
Spleen MRL | Kidney MRL | UUO | IRI | |
---|---|---|---|---|
N | 41 | 55 | 25 | 25 |
GM | 9.38 | 7.86 | 9.22 | 9.02 |
AM | 9.40 | 7.91 | 9.23 | 9.03 |
Minimum | 8.10 | 6.44 | 8.36 | 7.95 |
Maximum | 10.90 | 9.82 | 10.41 | 9.89 |
SD | 0.70 | 0.88 | 0.52 | 0.56 |
Variance | 0.48 | 0.77 | 0.27 | 0.31 |
CV | 0.05 | 0.06 | 0.03 | 0.03 |
Mouse right primer sequence | Mouse left primer sequence | Accession Nr. | Efficiency | Error value | |
---|---|---|---|---|---|
Dectin-1 | GTGCAGTAAGCTTTCCTGGG | TCCCGCAATCAGAGTGAAG | NM_020008 | 1.99 | 0.0007 |
MR1 | GTGGATTGTCTTGTGGAGCA | TTGTGGTGAGCTGAAAGGTG | NM_008625 | 2.16 | 0.0140 |
MR2 | CTCCAGACAGCCCTGCAT | GTCTTGCTTCTCGGGGGACT | NM_008626 | 1.81 | 0.0156 |
DC-SIGN | CTGCACAGTCTTCCTCTCCC | TGGTACTGGGTAGATGGTTCA | NM_133238 | 2.00 | 0.0188 |
Syk | TCTGCACCCCTTCAGAGTTC | TCCTTTCAACGTTCCATGCT | NM_011518 | 1.79 | 0.0510 |
Card-9 | ATGAGGCTGTGCCTGAGC | GCTGCAAGGACGAGAACTATG | NM_001037747 | 2.08 | 0.0174 |
Bcl-10 | TGCACGTAGATGATCAAAATGTC | ACGGAGGAGGATTTGACTGA | NM_009740 | 2.13 | 0.0740 |
Malt-1 | CAAAAGGATGTCCAGTTGCC | CACACTGAGGTTCTTCCGCT | NM_172833 | 1.82 | 0.0065 |
Src | TACCACTCCTCAGCCTGGAT | ACACGAGGAAGGTGGATGTC | NM_009271 | 2.19 | 0.0374 |
Dec-205 | TTCAGACCAATCCACAACCA | AGCTCACCTACCCAGCTTCA | NM_013825 | 2.03 | 0.1900 |
Galectin-1 | GCGAGGATTGAAGTGTAGGC | AATGTCTCAAAGTTCGGGGA | NM_008495 | 1.96 | 0.0085 |
Tim-3 | AGCCCATGTGGAAATTTTTG | CTCCAAGAACCCTAACCACG | NM_134250 | 2.00 | 0.0145 |
Trem-1 | CACTGTCAAAGTCTGGCCCT | ACTGCTGTGCGTGTTCTTTG | NM_021406 | 2.00 | 0.0740 |
DAP-12 | TTGCCTCTGTGTGTTGAGGT | CGGAAACAACACATTGCTGA | NM_011662 | 1.90 | 0.0379 |
Human right primer sequence | Human left primer sequence | Accession Nr | Efficiency | Error value | |
Dectin-1 | GGAGATGGGTTTTCTTGGGT | GACTGAGGTACCATGGCTCTG | NM_022570 | 2.06 | 0.0541 |
MR1 | CCCATCGGAATTTCTGTGAT | GGGTGCTGTTCTCCTACTGG | NM_002438 | 2.06 | 0.0587 |
MR2 | CAGTCCATGGCTGAAGATGA | GCTGCGTCCTGCTCCTC | NM_006039 | 2.22 | 0.1030 |
DC-SIGN | TTGTTGGGCTCTCCTCTGTT | AAGTAACCGCTTCACCTGGA | NM_021155 | 2.00 | 0.0122 |
Syk | AAAGAAGGGCAGGTGGTTG | GAGAGCGAGGAGGAGCG | NM_003177 | 2.07 | 0.0197 |
Card-9 | CTGTGCGTGCAGCTCCT | TCCAAGATGTACAAGGACCG | NM_052813 | 2.10 | 0.2160 |
Bcl-10 | TTGCACGTAGATGATCAAAATGT | TCCCTCACCGAGGAGGAC | NM_003921 | 2.00 | 0.0178 |
Malt-1 | GCCAAGACTGCCTTTGACTC | TTTCCTGCAGGCTATGGAAC | NM_006785 | 2.17 | 0.0364 |
Src | TAGTTGCTGGGGATGTAGCC | CTGTCCTTCAAGAAAGGCGA | NM_005417 | 2.12 | 0.0134 |
Dec-205 | CCAGCCAAAAACTTCTCATTT | TGGCTTCATGGGTCATGTTA | NM_002349 | 2.01 | 0.2010 |
Galectin-1 | AGGTTGTTGCTGTCTTTGCC | CAAACCTGGAGAGTGCCTTC | NM_002305 | 1.87 | 0.0810 |
Tim-3 | GCGAATTCCCTCTGCTACTG | CTTCGGCGCTTTAATTTTCA | NM_032782 | 2.02 | 0.0914 |
Trem-1 | TACTCAGGAATCCACCAGCC | CCGATGTCTCCACTCCTGAC | NM_018643 | 1.92 | 0.0731 |
DAP-12 | GTCATGATTCGGGCTCATTT | GAGACCGAGTCGCCTTATCA | NM_003332 | 2.08 | 0.0191 |
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Lech, M.; Susanti, H.E.; Römmele, C.; Gröbmayr, R.; Günthner, R.; Anders, H.-J. Quantitative Expression of C-Type Lectin Receptors in Humans and Mice. Int. J. Mol. Sci. 2012, 13, 10113-10131. https://doi.org/10.3390/ijms130810113
Lech M, Susanti HE, Römmele C, Gröbmayr R, Günthner R, Anders H-J. Quantitative Expression of C-Type Lectin Receptors in Humans and Mice. International Journal of Molecular Sciences. 2012; 13(8):10113-10131. https://doi.org/10.3390/ijms130810113
Chicago/Turabian StyleLech, Maciej, Heni Eka Susanti, Christoph Römmele, Regina Gröbmayr, Roman Günthner, and Hans-Joachim Anders. 2012. "Quantitative Expression of C-Type Lectin Receptors in Humans and Mice" International Journal of Molecular Sciences 13, no. 8: 10113-10131. https://doi.org/10.3390/ijms130810113