Protection against Ischemia-Induced Oxidative Stress Conferred by Vagal Stimulation in the Rat Heart: Involvement of the AMPK-PKC Pathway
Abstract
:1. Introduction
2. Results and Discussion
2.1. Vagal Stimulation Attenuated Myocardial Injury and Improved Cardiac Function
2.2. Changes of Serum Oxidant and Antioxidant Enzyme Activities in Each Group
2.3. Vagal Stimulation Inhibited Oxidative Stress in Rats with AMI Mainly via the NADPH Pathway
2.4 Effects of Vagal Stimulation on AMPK-PKC Pathway
3. Experimental Section
3.1. Animals
3.2. Heamodynamics Measurements and Induction of Acute Myocardial Infarction
3.3. Vagus Nerve Stimulation
3.4. Exclusion Criteria
3.5. Blood Sampling and Tissue Preparation
3.6. Cardiac Specific Injury Markers Measurement
3.7. Assessment of Infarction Size
3.8. Determination of Oxidant and Antioxidant Enzyme Activities
3.9. NADPH Oxidase Activity
3.10. Measurement of Superoxide Generation
3.11. Western Blotting Analysis
3.12. Statistical Analysis
4. Conclusions
Acknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
References
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Kong, S.-S.; Liu, J.-J.; Yu, X.-J.; Lu, Y.; Zang, W.-J. Protection against Ischemia-Induced Oxidative Stress Conferred by Vagal Stimulation in the Rat Heart: Involvement of the AMPK-PKC Pathway. Int. J. Mol. Sci. 2012, 13, 14311-14325. https://doi.org/10.3390/ijms131114311
Kong S-S, Liu J-J, Yu X-J, Lu Y, Zang W-J. Protection against Ischemia-Induced Oxidative Stress Conferred by Vagal Stimulation in the Rat Heart: Involvement of the AMPK-PKC Pathway. International Journal of Molecular Sciences. 2012; 13(11):14311-14325. https://doi.org/10.3390/ijms131114311
Chicago/Turabian StyleKong, Shan-Shan, Jin-Jun Liu, Xiao-Jiang Yu, Yi Lu, and Wei-Jin Zang. 2012. "Protection against Ischemia-Induced Oxidative Stress Conferred by Vagal Stimulation in the Rat Heart: Involvement of the AMPK-PKC Pathway" International Journal of Molecular Sciences 13, no. 11: 14311-14325. https://doi.org/10.3390/ijms131114311