Next Article in Journal
Chemical Composition and Antioxidant Activities of Three Polysaccharide Fractions from Pine Cones
Previous Article in Journal
Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(11), 14251-14261;

Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin

Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, Jiangsu, China
Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China
Author to whom correspondence should be addressed.
Received: 11 September 2012 / Revised: 16 October 2012 / Accepted: 29 October 2012 / Published: 5 November 2012
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [258 KB, uploaded 19 June 2014]


In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL−1, and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that β-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin. View Full-Text
Keywords: β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Cui, L.; Zhang, Z.-H.; Sun, E.; Jia, X.-B. Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin. Int. J. Mol. Sci. 2012, 13, 14251-14261.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top