Next Article in Journal
Effect of Freeze-Drying on the Antioxidant Compounds and Antioxidant Activity of Selected Tropical Fruits
Previous Article in Journal
Chitosan Interaction with Iron from Yoghurt Using an In Vitro Digestive Model: Comparative Study with Plant Dietary Fibers
Open AccessArticle

Sida rhomboidea. Roxb Leaf Extract Down-Regulates Expression of PPARγ2 and Leptin Genes in High Fat Diet Fed C57BL/6J Mice and Retards in Vitro 3T3L1 Pre-Adipocyte Differentiation

1
Division of Phytothrapeutics and Metabolic Endocrinology, Department of Zoology, The M. S. University of Baroda, Gujarat 390002, India
2
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agriculture University, Anand, Gujarat 388001, India
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2011, 12(7), 4661-4677; https://doi.org/10.3390/ijms12074661
Received: 3 May 2011 / Revised: 30 May 2011 / Accepted: 7 June 2011 / Published: 19 July 2011
(This article belongs to the Section Biochemistry)
Sida rhomboidea. Roxb leaf extract (SRLE) is being used by the populace of North-East India to alleviate symptoms of diabetes and obesity. We have previously reported its hypolipidemic and anti-diabetic properties. In this study, we report the effect of SRLE on (i) in vivo modulation of genes controlling high fat diet (HFD) induced obesity and (ii) in vitro 3T3L1 pre-adipocyte differentiation and leptin release. Supplementation with SRLE significantly prevented HFD induced increment in bodyweight, plasma lipids and leptin, visceral adiposity and adipocyte hypertrophy. Also, SRLE supplementation reduced food intake, down regulated PPARγ2, SREBP1c, FAS and LEP expressions and up-regulated CPT-1 in epididymal adipose tissue compared to obese mice. In vitro adipogenesis of 3T3L1 pre-adipocytes was significantly retarded in the presence of SRLE extract. Also decreased triglyceride accumulation, leptin release and glyceraldehyde-3-Phosphate dehydrogenase activity along with higher glycerol release without significant alteration of viability of 3T3L1 pre-adipocytes, was recorded. Our findings suggest that prevention of HFD induced visceral adiposity is primarily by down regulation of PPARγ2 and leptin gene expression coupled with attenuation of food intake in C57BL/6J mice. SRLE induced prevention of pre-adipocytes differentiation, and leptin release further substantiated these findings and scientifically validates the potential application of SRLE as a therapeutic agent against obesity. View Full-Text
Keywords: Sida rhomboidea. Roxb; obesity; 3T3L1 cells; PPARγ2; leptin Sida rhomboidea. Roxb; obesity; 3T3L1 cells; PPARγ2; leptin
Show Figures

Graphical abstract

MDPI and ACS Style

Thounaojam, M.C.; Jadeja, R.N.; Ramani, U.V.; Devkar, R.V.; Ramachandran, A.V. Sida rhomboidea. Roxb Leaf Extract Down-Regulates Expression of PPARγ2 and Leptin Genes in High Fat Diet Fed C57BL/6J Mice and Retards in Vitro 3T3L1 Pre-Adipocyte Differentiation. Int. J. Mol. Sci. 2011, 12, 4661-4677.

Show more citation formats Show less citations formats

Article Access Map

1
Only visits after 24 November 2015 are recorded.
Back to TopTop