Protective Effect of the Ethyl Acetate Fraction of Sargassum muticum against Ultraviolet B–Irradiated Damage in Human Keratinocytes
Abstract
:1. Introduction
2. Results
2.1. Scavenging Effect of SME toward Free Radicals
2.2. Effect of SME on Antioxidant Enzymes
2.3. Effect of SME against Cell Death Induced by UVB Radiation
3. Discussion
4. Experimental Section
4.1. Solvent Extraction of Sargassum Muticum
4.2. Reagents
4.3. Cell Culture
4.4. DPPH Radical Scavenging Activity
4.5. Intracellular ROS Scavenging Activity
4.6. Detection of Hydroxyl Radicals
4.7. Lipid Peroxidation Assay
4.8. Western Blot Analysis
4.9. SOD Activity
4.10. CAT Activity
4.11. Cell Viability Assay
4.12. Nuclear Staining with Hoechst 33342
4.13. Terminal Deoxynucleotidyl Transferase-Mediated Digoxigenin-dUTP Nick end Labeling (TUNEL) Assay
4.14. DNA Fragmentation
4.15. Statistical Analysis
5. Conclusion
Acknowledgements
References
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Piao, M.J.; Yoon, W.J.; Kang, H.K.; Yoo, E.S.; Koh, Y.S.; Kim, D.S.; Lee, N.H.; Hyun, J.W. Protective Effect of the Ethyl Acetate Fraction of Sargassum muticum against Ultraviolet B–Irradiated Damage in Human Keratinocytes. Int. J. Mol. Sci. 2011, 12, 8146-8160. https://doi.org/10.3390/ijms12118146
Piao MJ, Yoon WJ, Kang HK, Yoo ES, Koh YS, Kim DS, Lee NH, Hyun JW. Protective Effect of the Ethyl Acetate Fraction of Sargassum muticum against Ultraviolet B–Irradiated Damage in Human Keratinocytes. International Journal of Molecular Sciences. 2011; 12(11):8146-8160. https://doi.org/10.3390/ijms12118146
Chicago/Turabian StylePiao, Mei Jing, Weon Jong Yoon, Hee Kyoung Kang, Eun Sook Yoo, Young Sang Koh, Dong Sam Kim, Nam Ho Lee, and Jin Won Hyun. 2011. "Protective Effect of the Ethyl Acetate Fraction of Sargassum muticum against Ultraviolet B–Irradiated Damage in Human Keratinocytes" International Journal of Molecular Sciences 12, no. 11: 8146-8160. https://doi.org/10.3390/ijms12118146