Next Article in Journal
In Vitro Anti-Listerial Activities of Crude n-Hexane and Aqueous Extracts of Garcinia kola (heckel) Seeds
Next Article in Special Issue
Anti-UVC Irradiation and Metal Chelation Properties of 6-Benzoyl-5,7-dihydroxy-4-phenyl-chromen-2-one: An Implications for Anti-Cataract Agent
Previous Article in Journal
Evaluation of Antioxidant Compounds and Total Sugar Content in a Nectarine [Prunus persica (L.) Batsch] Progeny
Previous Article in Special Issue
Antioxidant Properties of the Edible Basidiomycete Armillaria mellea in Submerged Cultures
Open AccessArticle

N-Acetylcysteine Reduces Markers of Differentiation in 3T3-L1 Adipocytes

1
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires 1428, Argentina
2
Departamento de Química Inorgánica y Analítica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires 1428, Argentina
3
IBYME-CONICET, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2011, 12(10), 6936-6951; https://doi.org/10.3390/ijms12106936
Received: 30 August 2011 / Accepted: 10 October 2011 / Published: 19 October 2011
(This article belongs to the Special Issue Antioxidants)
Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP β) and peroxisomal proliferator activated receptor gamma (PPAR γ) expression; we suggested that intracellular GSH content could be responsible for these effects. View Full-Text
Keywords: NAC; triglyceride; adipocyte differentiation NAC; triglyceride; adipocyte differentiation
MDPI and ACS Style

Calzadilla, P.; Sapochnik, D.; Cosentino, S.; Diz, V.; Dicelio, L.; Calvo, J.C.; Guerra, L.N. N-Acetylcysteine Reduces Markers of Differentiation in 3T3-L1 Adipocytes. Int. J. Mol. Sci. 2011, 12, 6936-6951.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop