Next Article in Journal
Mutagenicity of New Lead Compounds to Treat Sickle Cell Disease Symptoms in a Salmonella/Microsome Assay
Next Article in Special Issue
Miniaturization in Biocatalysis
Previous Article in Journal
Lipid Vesicle Aggregation Induced by Cooling
Open AccessArticle

Covalent Anchoring of Chloroperoxidase and Glucose Oxidase on the Mesoporous Molecular Sieve SBA-15

Erlangen Catalysis Resource Center, Universität Erlangen Nürnberg, Egerlandstr. 3, 91058 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2010, 11(2), 762-778; https://doi.org/10.3390/ijms11020762
Received: 4 January 2010 / Revised: 9 February 2010 / Accepted: 9 February 2010 / Published: 24 February 2010
(This article belongs to the Special Issue Biocatalysis)
Functionalization of porous solids plays an important role in many areas, including heterogeneous catalysis and enzyme immobilization. In this study, large-pore ordered mesoporous SBA-15 molecular sieves were synthesized with tetraethyl orthosilicate (TEOS) in the presence of the non-ionic triblock co-polymer Pluronic P123 under acidic conditions. These materials were grafted with 3 aminopropyltrimethoxysilane (ATS), 3-glycidoxypropyltrimethoxysilane (GTS) and with 3 aminopropyltrimethoxysilane and glutaraldehyde (GA-ATS) in order to provide covalent anchoring points for enzymes. The samples were characterized by nitrogen adsorption, powder X-ray diffraction, solid-state NMR spectroscopy, elemental analysis, diffuse reflectance fourier transform infrared spectroscopy and diffuse reflectance UV/Vis spectroscopy. The obtained grafted materials were then used for the immobilization of chloroperoxidase (CPO) and glucose oxidase (GOx) and the resulting biocatalysts were tested in the oxidation of indole. It is found that enzymes anchored to the mesoporous host by the organic moieties can be stored for weeks without losing their activity. Furthermore, the covalently linked enzymes are shown to be less prone to leaching than the physically adsorbed enzymes, as tested in a fixed-bed reactor under continuous operation conditions. View Full-Text
Keywords: functionalization; mesoporous silica; enzyme immobilization functionalization; mesoporous silica; enzyme immobilization
Show Figures

Graphical abstract

MDPI and ACS Style

Jung, D.; Streb, C.; Hartmann, M. Covalent Anchoring of Chloroperoxidase and Glucose Oxidase on the Mesoporous Molecular Sieve SBA-15. Int. J. Mol. Sci. 2010, 11, 762-778.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop