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Open AccessArticle

Protein and Metabolite Analysis Reveals Permanent Induction of Stress Defense and Cell Regeneration Processes in a Tobacco Cell Suspension Culture

Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06 466, Gatersleben, Germany
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Int. J. Mol. Sci. 2009, 10(7), 3012-3032; https://doi.org/10.3390/ijms10073012
Received: 8 June 2009 / Revised: 26 June 2009 / Accepted: 26 June 2009 / Published: 6 July 2009
(This article belongs to the Special Issue Biotic and Abiotic Stress)
The secretome of a tobacco cell suspension culture was investigated by a combined proteomic and metabolomic approach. Protein analysis from 2-DE gels led to identification of 32 out of 60 spots from culture medium. Identified proteins were mainly involved in stress defence and cell regeneration processes. Among them three putative new isoforms, e.g. for chitinase, peroxidase and β-1,4-xylosidase were identified, not yet present in available protein databases for the genus Nicotiana. GC-MS analysis of time course experiments revealed significant changes for metabolites involved in energy transport, signalling and cell development. Among them, the most significant increase was found for putrescine in the medium of cultures entering the exponential phase. Results showed strong abundance of stress associated proteins and metabolites in the absence of elicitors or additional stress treatments. View Full-Text
Keywords: apoplast; suspension culture; tobacco; extracellular proteins; secreted putrescine apoplast; suspension culture; tobacco; extracellular proteins; secreted putrescine
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Lippmann, R.; Kaspar, S.; Rutten, T.; Melzer, M.; Kumlehn, J.; Matros, A.; Mock, H.-P. Protein and Metabolite Analysis Reveals Permanent Induction of Stress Defense and Cell Regeneration Processes in a Tobacco Cell Suspension Culture. Int. J. Mol. Sci. 2009, 10, 3012-3032.

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