An accessible and easy-to-read text for students requiring a broad overview of the key techniques used to characterise the structure and function of complex biomacromolecules such as proteins and DNA. It bridges the gap between general biochemistry textbooks and the more specialist texts covering individual techniques.
Topics covered include chromatography, spectroscopy, mass spectrometry, electrophoresis, X-ray diffraction, centrifugation and biocalorimetry. New developments are placed in context by describing the physical principles on which they depend, examining the range of biophysical applications most widely used and, emphasising the overall similarities of experimental approach.
Written by a biochemist with extensive teaching experience, the book:
describes the advantages and disadvantages of each technique and compares one technique to another;
introduces experimental approaches in a non-mathematical way, using practical examples; and
provides a bibliography, including useful web sites, at the end of each chapter.
This book will be invaluable to undergraduates and postgraduates studying biochemistry, molecular biology and related disciplines.
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