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Volume 31
Issue 12
10.3390/molecules31122075
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Study on Regulatory Mechanism of Gastrodia elata Specific microRNA Targeting JNK3 in Alzheimer’s Disease

by
Hongyao Li
1,†,
Zhongteng Lu
1,†,
Ke Gao
1,
Jianjun Guo
1,
Zuoming Nie
1,2 and
Qing Sheng
1,*
1
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
2
Shaoxing Academy of Biomedicine, Zhejiang Sci-Tech University, Shaoxing 312000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2026, 31(12), 2075; https://doi.org/10.3390/molecules31122075 (registering DOI)
Submission received: 4 April 2026 / Revised: 6 June 2026 / Accepted: 9 June 2026 / Published: 12 June 2026
(This article belongs to the Section Medicinal Chemistry)
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Abstract

Alzheimer’s disease (AD) is characterized by Tau hyperphosphorylation, β-amyloid (Aβ) accumulation, and progressive neuronal loss. Gastrodia elata (G. elata), a traditional Chinese medicine with well-established neuroprotective properties, was investigated. Two G. elata-derived miRNAs, Gas-miR04-3p and Gas-miR19-5p, were identified as regulators of JNK3. By means of Western blot, RT-qPCR, and assessments of antioxidant indices, it was demonstrated that Gas-miR04-3p and Gas-miR19-5p can suppress JNK3 expression, reduce Tau phosphorylation at Ser202 and Ser396, enhance antioxidant capacity, and attenuate apoptosis in AD-related cellular and molecular pathology models. These miRNAs were also detectable in murine brain tissues following oral administration of total RNA extracted from G. elata. Their administration was associated with decreased JNK3 activation, alleviated Tau hyperphosphorylation, and improved expression of apoptosis-related proteins in AD mouse models. These results suggest that G. elata miRNAs may exert neuroprotective effects through regulation of JNK3 signaling, thereby attenuating Tau-related pathological changes and neuronal injury in AD-related models.
Keywords: Alzheimer’s disease; Gastrodia elata miRNA; cross-kingdom; JNK3; tau hyperphosphorylation Alzheimer’s disease; Gastrodia elata miRNA; cross-kingdom; JNK3; tau hyperphosphorylation
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MDPI and ACS Style

Li, H.; Lu, Z.; Gao, K.; Guo, J.; Nie, Z.; Sheng, Q. Study on Regulatory Mechanism of Gastrodia elata Specific microRNA Targeting JNK3 in Alzheimer’s Disease. Molecules 2026, 31, 2075. https://doi.org/10.3390/molecules31122075

AMA Style

Li H, Lu Z, Gao K, Guo J, Nie Z, Sheng Q. Study on Regulatory Mechanism of Gastrodia elata Specific microRNA Targeting JNK3 in Alzheimer’s Disease. Molecules. 2026; 31(12):2075. https://doi.org/10.3390/molecules31122075

Chicago/Turabian Style

Li, Hongyao, Zhongteng Lu, Ke Gao, Jianjun Guo, Zuoming Nie, and Qing Sheng. 2026. "Study on Regulatory Mechanism of Gastrodia elata Specific microRNA Targeting JNK3 in Alzheimer’s Disease" Molecules 31, no. 12: 2075. https://doi.org/10.3390/molecules31122075

APA Style

Li, H., Lu, Z., Gao, K., Guo, J., Nie, Z., & Sheng, Q. (2026). Study on Regulatory Mechanism of Gastrodia elata Specific microRNA Targeting JNK3 in Alzheimer’s Disease. Molecules, 31(12), 2075. https://doi.org/10.3390/molecules31122075

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