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Volume 30
Issue 17
10.3390/molecules30173551
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Stable Gold@Polydopamine@ssDNA Bioconjugates for Highly Efficient Detection of Tumor-Related mRNA in Living Cells

by
Senhao Hu
†,
Wenjing Wang
†,
Yu Zou
,
Chunmei Li
,
Hongyan Zou
*,
Chengzhi Huang
and
Lei Zhan
*
Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2025, 30(17), 3551; https://doi.org/10.3390/molecules30173551
Submission received: 8 July 2025 / Revised: 27 August 2025 / Accepted: 28 August 2025 / Published: 29 August 2025
Versions Notes

Abstract

The development of low-background, facile, and robust fluorescent nanoprobes for imaging and monitoring of intracellular mRNA changes remains a great challenge. Taking advantage of the high fluorescence quenching efficiency of core-shell gold@polydopamine (Au@PDA) nanocomposites and Ca2+-promoting DNA adsorption stability, a simple and universal bioconjugate strategy was designed to a construct fluorescent nanoprobe for highly efficient tumor-related mRNA imaging. The fluorescence of Cy5-labeled DNA was quenched up to 92.38% by the AuNP and PDA via nanometal surface energy transfer (NSET) and photoinduced electron transfer (PET), respectively. TK1 mRNA, a biomarker of tumor growth, initiates hybridization and results in fluorescence recovery, which built the foundation for identifying the expression level changes in living cells. More importantly, three kinds of tumor-related mRNA (TK1 mRNA, GalNAc-T mRNA, and C-myc mRNA) can be detected simultaneously with different fluorophore-modified recognition sequences, which can avoid false positive signals and improve the reliability of cancer diagnostic, holding great promise for cancer diagnosis, prognosis, and therapy.
Keywords: Au@PDA bioconjugates; tumor-related mRNA; Ca2+-assisted DNA adsorption; multiplex imaging Au@PDA bioconjugates; tumor-related mRNA; Ca2+-assisted DNA adsorption; multiplex imaging

Share and Cite

MDPI and ACS Style

Hu, S.; Wang, W.; Zou, Y.; Li, C.; Zou, H.; Huang, C.; Zhan, L. Stable Gold@Polydopamine@ssDNA Bioconjugates for Highly Efficient Detection of Tumor-Related mRNA in Living Cells. Molecules 2025, 30, 3551. https://doi.org/10.3390/molecules30173551

AMA Style

Hu S, Wang W, Zou Y, Li C, Zou H, Huang C, Zhan L. Stable Gold@Polydopamine@ssDNA Bioconjugates for Highly Efficient Detection of Tumor-Related mRNA in Living Cells. Molecules. 2025; 30(17):3551. https://doi.org/10.3390/molecules30173551

Chicago/Turabian Style

Hu, Senhao, Wenjing Wang, Yu Zou, Chunmei Li, Hongyan Zou, Chengzhi Huang, and Lei Zhan. 2025. "Stable Gold@Polydopamine@ssDNA Bioconjugates for Highly Efficient Detection of Tumor-Related mRNA in Living Cells" Molecules 30, no. 17: 3551. https://doi.org/10.3390/molecules30173551

APA Style

Hu, S., Wang, W., Zou, Y., Li, C., Zou, H., Huang, C., & Zhan, L. (2025). Stable Gold@Polydopamine@ssDNA Bioconjugates for Highly Efficient Detection of Tumor-Related mRNA in Living Cells. Molecules, 30(17), 3551. https://doi.org/10.3390/molecules30173551

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