Trametes hirsuta as an Attractive Biocatalyst for the Preparative Scale Biotransformation of Isosafrole into Piperonal
Abstract
:1. Introduction
2. Results and Discussion
2.1. Screening Scale Biotransformations
2.2. The Influence of Carbon and Nitrogen Source on Biotransformation
2.3. Preparative Biotransformations of Isosafrole (1a) to Piperonal (1b)
2.4. Species Identification
3. Materials and Methods
3.1. Materials
3.2. Microorganisms
3.3. Fungi Isolation Methods
3.4. Molecular Identification of Fungal Strains
3.5. Screening Scale Biotransformations
3.6. Modification of Culture Media Composition
3.7. Extraction Procedure
3.8. Preparative Biotransformations
3.9. Analysis Procedure
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Strain | Time (Days) | Substrate (%) 1a | Products (%) | |
---|---|---|---|---|
1b | 1c | |||
Laetiporus sulphurens AM515 | 7 | 0 | 0 | 100 |
14 | 0 | 0 | 100 | |
Mortierella isabelina AM212 | 7 | 100 | 0 | 0 |
14 | 87 | 13 | 0 | |
Piptoporus betulinus AM40 | 7 | 23 | 0 | 77 |
14 | 17 | 0 | 83 | |
Trametes versicolor AM536 | 7 | 63 | 10 | 27 |
14 | 36 | 14 | 50 | |
Trametes hirsuta d28 | 7 | 79 | 8 | 13 |
14 | 42 | 10 | 48 |
Source | Time (Days) | Substrate (%) 1a | Products (%) | ||
---|---|---|---|---|---|
Carbon | Nitrogen | 1b | 1c | ||
Fructose | Peptone | 7 | 44 | 3 | 53 |
14 | 31 | 3 | 66 | ||
Mannose | 7 | 38 | 3 | 59 | |
14 | 29 | 3 | 68 | ||
Ribose | 7 | 33 | 14 | 53 | |
14 | 18 | 18 | 64 | ||
Starch | 7 | 52 | 2 | 46 | |
14 | 34 | 4 | 62 | ||
Galactose | 7 | 62 | 0 | 38 | |
14 | 14 | 0 | 86 | ||
Glucose | Casein hydrolysate | 7 | 19 | 33 | 48 |
14 | 7 | 28 | 65 | ||
Lactalbumin hydrolysate | 7 | 17 | 10 | 73 | |
14 | 17 | 10 | 73 | ||
Meat peptone | 7 | 51 | 15 | 34 | |
14 | 61 | 14 | 25 | ||
Potatoes peptone | 7 | 83 | 3 | 14 | |
14 | 65 | 4 | 31 | ||
Soy peptone | 7 | 51 | 3 | 46 | |
14 | 32 | 4 | 64 |
Strain | Source | Time (Days) | Substrate (%) 1a | Products (%) | ||
---|---|---|---|---|---|---|
Carbon | Nitrogen | 1b | 1c | |||
Trametes versicolor AM536 | Glucose | Peptone | 7 | 40 | 20 | 40 |
14 | 34 | 24 | 42 | |||
Ribose | Peptone | 7 | 72 | 0 | 28 | |
14 | 53 | 16 | 31 | |||
Ribose | Casein hydrolysate | 7 | 57 | 5 | 38 | |
14 | 45 | 16 | 39 | |||
Glucose | Casein hydrolysate | 7 | 47 | 13 | 40 | |
14 | 32 | 20 | 48 | |||
Trametes hirsuta d28 | Glucose | Peptone | 7 | 33 | 35 | 32 |
14 | 11 | 43 | 46 | |||
Ribose | Peptone | 7 | 100 | 0 | 0 | |
14 | 100 | 0 | 0 | |||
Ribose | Casein hydrolysate | 7 | 100 | 0 | 0 | |
14 | 100 | 0 | 0 | |||
Glucose | Casein hydrolysate | 7 | 17 | 32 | 51 | |
14 | 10 | 33 | 57 |
Strain | Time (Days) | Substrate (%) 1a | Products (%) | |
---|---|---|---|---|
1b | 1c | |||
T. hirsuta Th1_1 | 7 | 68 | 0 | 32 |
14 | 66 | 0 | 34 | |
T. hirsuta Th2_2 | 7 | 24 | 23 | 53 |
14 | 8 | 38 | 54 | |
T. hirsuta Th5_2 | 7 | 36 | 0 | 64 |
14 | 22 | 0 | 78 | |
T. hirsuta Th5_3 | 7 | 33 | 0 | 67 |
14 | 34 | 0 | 66 |
Strain | Substrate (%) 1a | Products (%) | Isolation Yield of 1b (%) | Amount of 1b Obtained (mg) | |
---|---|---|---|---|---|
1b | 1c | ||||
T. hirsuta Th2_2 | 11 | 82 | 7 | 62 | 124 |
T. hirsuta d28 | 25 | 69 | 6 | 50.5 | 101 |
Biotransformation Approach | Biocatalysts | Substrate | Conversion (%) | Obtained Amount | Ref. |
---|---|---|---|---|---|
Whole cells | Trametes hirsuta | Isosafrole | 4 | np * | [8] |
Whole cells | Cladosporium sphaerospermum | Isosafrole | 9.8 | np * | [6] |
Post-culture medium | Paecilomyces variotii | Isosafrole | 20 | np * | [2] |
Whole cells | Serratia liquefaciens | Isosafrole | 38.5 | 282.32 mg/L ** | [7] |
GMO co-expressing system | Escherichia coli (FDH and TAO3G2) | Isosafrole | 96.02 | 19.45 g/L | [22] |
Purified enzyme | POAA2 from Pleurotus eryngii P34 | Piperonylic alcohol | 95 | 244.6 mg | [11] |
GMO co-expressing system | E. coli (CAR from Neurospora crassa) | Piperonylic acid | 100 | 1.66 g *** | [10] |
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Hernik, D.; Szczepańska, E.; Brenna, E.; Patejuk, K.; Olejniczak, T.; Strzała, T.; Boratyński, F. Trametes hirsuta as an Attractive Biocatalyst for the Preparative Scale Biotransformation of Isosafrole into Piperonal. Molecules 2023, 28, 3643. https://doi.org/10.3390/molecules28083643
Hernik D, Szczepańska E, Brenna E, Patejuk K, Olejniczak T, Strzała T, Boratyński F. Trametes hirsuta as an Attractive Biocatalyst for the Preparative Scale Biotransformation of Isosafrole into Piperonal. Molecules. 2023; 28(8):3643. https://doi.org/10.3390/molecules28083643
Chicago/Turabian StyleHernik, Dawid, Ewa Szczepańska, Elisabetta Brenna, Katarzyna Patejuk, Teresa Olejniczak, Tomasz Strzała, and Filip Boratyński. 2023. "Trametes hirsuta as an Attractive Biocatalyst for the Preparative Scale Biotransformation of Isosafrole into Piperonal" Molecules 28, no. 8: 3643. https://doi.org/10.3390/molecules28083643