Next Article in Journal
Procyanidins Alleviated Cerebral Ischemia/Reperfusion Injury by Inhibiting Ferroptosis via the Nrf2/HO-1 Signaling Pathway
Next Article in Special Issue
Thiocholine-Mediated One-Pot Peptide Ligation and Desulfurization
Previous Article in Journal
Biochemical Profile and In Vitro Therapeutic Properties of Two Euhalophytes, Halocnemum strobilaceum Pall. and Suaeda fruticosa (L.) Forske., Grown in the Sabkha Ecosystem in the Algerian Sahara
Previous Article in Special Issue
Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 28
Issue 8
10.3390/molecules28083581
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells

by
Alexey S. Nagel
1,†,
Natalia V. Rudenko
2,*,†,
Polina N. Luchkina
1,
Anna P. Karatovskaya
2,
Anna V. Zamyatina
2,
Zhanna I. Andreeva-Kovalevskaya
1,
Alexander V. Siunov
1,
Fedor A. Brovko
2 and
Alexander S. Solonin
1
1
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences, 5 Prospekt Nauki, 142290 Pushchino, Moscow Region, Russia
2
Pushchino Branch, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 6 Prospekt Nauki, 142290 Pushchino, Moscow Region, Russia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2023, 28(8), 3581; https://doi.org/10.3390/molecules28083581
Submission received: 10 March 2023 / Revised: 28 March 2023 / Accepted: 18 April 2023 / Published: 19 April 2023
(This article belongs to the Special Issue Peptide and Protein Chemistry: Design, Synthesis, and Applications)
Browse Figures
Versions Notes

Abstract

Hemolysin II (HlyII) is one of the virulence factors of the opportunistic bacterium Bacillus cereus belonging to the group of β-pore-forming toxins. This work created a genetic construct encoding a large C-terminal fragment of the toxin (HlyIILCTD, M225–I412 according to the numbering of amino acid residues in HlyII). A soluble form of HlyIILCTD was obtained using the SlyD chaperone protein. HlyIILCTD was first shown to be capable of agglutinating rabbit erythrocytes. Monoclonal antibodies against HlyIILCTD were obtained by hybridoma technology. We also proposed a mode of rabbit erythrocyte agglutination by HlyIILCTD and selected three anti-HlyIILCTD monoclonal antibodies that inhibited the agglutination.
Keywords: pore-forming toxin; monoclonal antibody; agglutination; three-dimensional protein structure modeling pore-forming toxin; monoclonal antibody; agglutination; three-dimensional protein structure modeling

Share and Cite

MDPI and ACS Style

Nagel, A.S.; Rudenko, N.V.; Luchkina, P.N.; Karatovskaya, A.P.; Zamyatina, A.V.; Andreeva-Kovalevskaya, Z.I.; Siunov, A.V.; Brovko, F.A.; Solonin, A.S. Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells. Molecules 2023, 28, 3581. https://doi.org/10.3390/molecules28083581

AMA Style

Nagel AS, Rudenko NV, Luchkina PN, Karatovskaya AP, Zamyatina AV, Andreeva-Kovalevskaya ZI, Siunov AV, Brovko FA, Solonin AS. Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells. Molecules. 2023; 28(8):3581. https://doi.org/10.3390/molecules28083581

Chicago/Turabian Style

Nagel, Alexey S., Natalia V. Rudenko, Polina N. Luchkina, Anna P. Karatovskaya, Anna V. Zamyatina, Zhanna I. Andreeva-Kovalevskaya, Alexander V. Siunov, Fedor A. Brovko, and Alexander S. Solonin. 2023. "Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells" Molecules 28, no. 8: 3581. https://doi.org/10.3390/molecules28083581

APA Style

Nagel, A. S., Rudenko, N. V., Luchkina, P. N., Karatovskaya, A. P., Zamyatina, A. V., Andreeva-Kovalevskaya, Z. I., Siunov, A. V., Brovko, F. A., & Solonin, A. S. (2023). Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells. Molecules, 28(8), 3581. https://doi.org/10.3390/molecules28083581

Article Metrics

Back to TopTop