Cell-Penetrating Peptides for Use in Development of Transgenic Plants
Round 1
Reviewer 1 Report
The review article entitled “Cell-Penetrating Peptides for Use in Development of Trans- 2 genic Plants” addressing the nice contents, Figs. resolution is very high however English used in the text is very poor and need to revised thoroughly.
Few examples are as under,
1- In introduction section paragraph on line 39-41 Modern agriculture is facing major……..may be revised and concise as the word “and” has been used about 5 times in same paragraph
2- Various references are too old i.e., references from 19th that may be updated
3- While writing Scientific names of plant species once may be write the complete name then abbreviations but the authors have written full names repeatedly in tables as well as in text i.e., ‘’Arabidopsis thaliana’’.
4- Similar writing pattern has been used for microorganism’s names such as ‘’Agrobacterium tumefaciens’’ on line number 20, 53, 114 that need to be revised and check throughout the manuscript.
5- List of genes may be added that indicating the genes that were successfully achieved for Development of Trans- 2 genic Plants.
6- Conclusion section may be improved by mentioning key findings of this review.
Author Response
We appreciate your critical review and have carefully revised the manuscript according to your comments. Our revised manuscript has been significantly edited by a native English-speaking colleague. Your major comments are answered below:
- We have rephrased this sentence according to your suggestions in a more clear manner.
- Yes, we have updated both 18th and 19th
- Accordingly, we have checked and corrected scientific names of plant species in text and tables.
- As stated above, we have corrected these names throughout the entire manuscript.
- We have followed your comments to add a new Table 2 illustrating the genes used in transgenic plants.
- As suggested, we have re-written the Conclusion section in a more conclusive manner to include key findings of this review.
A detailed list of changes in the revised manuscript marked in red color is summarized below.
A list of changes:
Line
Page* number Original version Revised version
1 7 College of Acience and College of Science and
1 17 with improved gains in yield… with improved yield…
1 18 biotechnology that introduces… biotechnology introducing…
1 32 In sum, the… In summary, the…
1 38 Crop yield trends… The current trends in…
1 40 and plant pests... plant pests...
1 41 and all of ... All of ...
2 52 in 1983 with an... in 1983 when an...
2 53 Agrobacterium tumefaciens A. tumefaciens
2 59 plants in either a ... plants either directly ...
2 60 Another way of ... Additional gene delivery...
2 68 tissue damages and tissue damage and
2 98 embryos were also served embryos also served
3 104 The pros of ... The benefits of …
3 109 ions with electrostatics, ions via electrostatics,
3 112 reduce the zeal of reduce the potential of
3 114 Agrobacterium tumefaciens A. tumefaciens
3 116 deliver genes into host… deliver genes into the host…
3 121 Besides, it took long Additionally, it took long
3 126 non-viral but peptide-based gene non-viral peptide-based gene...
3 130 to detailly introduce... to introduce...in detail.
3 133 Cell membrane is a The cell membrane is a
3 138 nucleic acids into nucleic acids, into
3 142 improved while CPPs improved when CPPs
3 148 Till now, studies Until now, studies
4 163 It further offered... Additionally, it offered...
7 210 These are the pros of... As shown, there are...
7 211 However, the cons come Unfortunately, the cons come
7 216 understanding about entry understanding their entry
8 255 membrane with electrostatics membrane utilizing electrostatics
8 263 while change the membrane... while changing the membrane...
8 280 [22,63]. However, SR9 [22,63], however, SR9
9 308 either leads to thin the either thins the
9 328 Kauffman et al. suggested The authors also suggested
9 340 However, a good transgenic A good transgenic
9 347 [100]. Here, we... [100]. Various DNA...
9 355 It enters nucleus not NLS enters nucleus not
10 372 3.2. Chloroplasts... Table 2...
10 384 and is easy to be manufactured and is easily manufactured
10 396 sequence is also following the sequence also follows the
10 399 and transfected the plasmid transfecting the plasmid
11 411 This characteristic attack... This characteristic attacks...
11 418 which is able to be recognized which is recognized
11 425 of Arabidopsis thaliana of A. thaliana
11 440 agriculture. Varying... agriculture. In addition, ...
11 442 By targeting to nuclei By targeting nuclei
11 455 Acknowledgments: None. Acknowledgments: We...
12 486 Agrobacterium- and... Agrobacterium- and...
12 496 18. Potrykus, I. 18. Davey, M.R.; ...
12 497 19. Finer, K.R.; ... 19. Hwang, H.H.; ...
13 550 In vivo proof of ... In vivo proof of ...
14 600 on Chlamydomonas on Chlamydomonas ...
14 623 of Arabidopsis thaliana of Arabidopsis thaliana
15 644 In silico approaches In silico approaches
*Page and line numbers are referred to the original version.
Reviewer 2 Report
The article "Cell-Penetrating Peptides in Plant Delivery Systems: State of the Art and Future Directions" provides a comprehensive review of the use of cell-penetrating peptides (CPPs) in plant gene delivery systems. The authors discuss the current state of CPP-based gene delivery systems and their potential for use in agriculture. They describe the different types of CPPs and their cellular entry mechanisms, as well as modifications that can be made to CPPs for specific organelle-targeted delivery in plant cells. The authors also provide a brief overview of other gene delivery methods used in plants. Overall, this article provides valuable information for researchers interested in developing gene delivery systems using CPPs for plant biotechnology. Based on the detailed and thorough analysis presented in this article, I would like to recommend its acceptance for publication.
Minor concern:
Line 129: “We devote the sections below to detailly introduce CPPs and their roles in transgenic plants” to “We devote the sections below to introduce CPPs and their roles in transgenic plants in detail.”
Author Response
Thank you for the detailed review. We have carefully revised the original manuscript according to your valuable comments. Our answer to your minor concern is listed below:
- Line 129: We have rephrased this sentence according to your suggestion.
A detailed list of changes in the revised manuscript marked in red color is summarized below.
A list of changes:
Line
Page* number Original version Revised version
1 7 College of Acience and College of Science and
1 17 with improved gains in yield… with improved yield…
1 18 biotechnology that introduces… biotechnology introducing…
1 32 In sum, the… In summary, the…
1 38 Crop yield trends… The current trends in…
1 40 and plant pests... plant pests...
1 41 and all of ... All of ...
2 52 in 1983 with an... in 1983 when an...
2 53 Agrobacterium tumefaciens A. tumefaciens
2 59 plants in either a ... plants either directly ...
2 60 Another way of ... Additional gene delivery...
2 68 tissue damages and tissue damage and
2 98 embryos were also served embryos also served
3 104 The pros of ... The benefits of …
3 109 ions with electrostatics, ions via electrostatics,
3 112 reduce the zeal of reduce the potential of
3 114 Agrobacterium tumefaciens A. tumefaciens
3 116 deliver genes into host… deliver genes into the host…
3 121 Besides, it took long Additionally, it took long
3 126 non-viral but peptide-based gene non-viral peptide-based gene...
3 130 to detailly introduce... to introduce...in detail.
3 133 Cell membrane is a The cell membrane is a
3 138 nucleic acids into nucleic acids, into
3 142 improved while CPPs improved when CPPs
3 148 Till now, studies Until now, studies
4 163 It further offered... Additionally, it offered...
7 210 These are the pros of... As shown, there are...
7 211 However, the cons come Unfortunately, the cons come
7 216 understanding about entry understanding their entry
8 255 membrane with electrostatics membrane utilizing electrostatics
8 263 while change the membrane... while changing the membrane...
8 280 [22,63]. However, SR9 [22,63], however, SR9
9 308 either leads to thin the either thins the
9 328 Kauffman et al. suggested The authors also suggested
9 340 However, a good transgenic A good transgenic
9 347 [100]. Here, we... [100]. Various DNA...
9 355 It enters nucleus not NLS enters nucleus not
10 372 3.2. Chloroplasts... Table 2...
10 384 and is easy to be manufactured and is easily manufactured
10 396 sequence is also following the sequence also follows the
10 399 and transfected the plasmid transfecting the plasmid
11 411 This characteristic attack... This characteristic attacks...
11 418 which is able to be recognized which is recognized
11 425 of Arabidopsis thaliana of A. thaliana
11 440 agriculture. Varying... agriculture. In addition, ...
11 442 By targeting to nuclei By targeting nuclei
11 455 Acknowledgments: None. Acknowledgments: We...
12 486 Agrobacterium- and... Agrobacterium- and...
12 496 18. Potrykus, I. 18. Davey, M.R.; ...
12 497 19. Finer, K.R.; ... 19. Hwang, H.H.; ...
13 550 In vivo proof of ... In vivo proof of ...
14 600 on Chlamydomonas on Chlamydomonas ...
14 623 of Arabidopsis thaliana of Arabidopsis thaliana
15 644 In silico approaches In silico approaches
*Page and line numbers are referred to the original version.
Round 2
Reviewer 1 Report
Authors have incorporated the all the mentioned garamatical/technical errors and revised version is fine to accept
