Gastrodin and Gastrodigenin Improve Energy Metabolism Disorders and Mitochondrial Dysfunction to Antagonize Vascular Dementia
Abstract
:1. Introduction
2. Results
2.1. Gas and HBA Improve Behavioral and Cognitive Alterations in VD Rats
2.2. Gas and HBA Reduce Attenuates 2-VO-Induced Neuronal Damage in the Hippocampus
2.3. Gas and HBA Reduce Aβ and Tau Protein Expression in the Brain of VD Rats
2.4. Gas and HBA Inhibit the Changes in Energy Metabolism in VD Rats
2.5. Gas and HBA Protect HT-22 Cells from H2O2-Induced Damage
2.6. Gas and HBA Attenuated Mitochondrial Dysfunction in HT-22 Cells Induced by H2O2
3. Material and Method
3.1. Chemicals and Materials
3.2. Animals
3.3. VD Rats Model Preparation
3.4. Morris Water Maze Test
3.5. Passive Avoidance Test
3.6. Brain Tissue Staining
3.6.1. Hematoxylin and Eosin (H&E) Staining
3.6.2. Nissl’s Staining
3.6.3. Immunohistochemical Staining (IHC)
3.7. P-Tau217 ELISA Test
3.8. Absolute Quantification of Targeted Energy Metabolism
3.9. Cell Culture
3.10. MTT Assay for Cell Viability
3.11. Mitochondrial Respiration Assay
3.12. Statistical Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Wu, S.; Huang, R.; Zhang, R.; Xiao, C.; Wang, L.; Luo, M.; Song, N.; Zhang, J.; Yang, F.; Liu, X.; et al. Gastrodin and Gastrodigenin Improve Energy Metabolism Disorders and Mitochondrial Dysfunction to Antagonize Vascular Dementia. Molecules 2023, 28, 2598. https://doi.org/10.3390/molecules28062598
Wu S, Huang R, Zhang R, Xiao C, Wang L, Luo M, Song N, Zhang J, Yang F, Liu X, et al. Gastrodin and Gastrodigenin Improve Energy Metabolism Disorders and Mitochondrial Dysfunction to Antagonize Vascular Dementia. Molecules. 2023; 28(6):2598. https://doi.org/10.3390/molecules28062598
Chicago/Turabian StyleWu, Sha, Rong Huang, Ruiqin Zhang, Chuang Xiao, Lueli Wang, Min Luo, Na Song, Jie Zhang, Fang Yang, Xuan Liu, and et al. 2023. "Gastrodin and Gastrodigenin Improve Energy Metabolism Disorders and Mitochondrial Dysfunction to Antagonize Vascular Dementia" Molecules 28, no. 6: 2598. https://doi.org/10.3390/molecules28062598