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Volume 28
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10.3390/molecules28196887
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Article

Antioxidant Peptides from the Collagen of Antler Ossified Tissue and Their Protective Effects against H2O2-Induced Oxidative Damage toward HaCaT Cells

by
Xi Chen
1,†,
Peijun Xia
1,†,
Shuo Zheng
1,
Yi Li
1,
Jiayuan Fang
1,
Ze Ma
1,
Libo Zhang
1,
Xunming Zhang
1,
Linlin Hao
1,* and
Hong Zhang
2,*
1
College of Animal Science, Jilin University, 5333 Xi’an Road, Changchun 130062, China
2
Hospital of Stomatology, Jilin University, Changchun 130015, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2023, 28(19), 6887; https://doi.org/10.3390/molecules28196887
Submission received: 31 July 2023 / Revised: 14 September 2023 / Accepted: 26 September 2023 / Published: 30 September 2023
(This article belongs to the Topic Antioxidant Activity of Natural Products)
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Abstract

Antler ossified tissue has been widely used for the extraction of bioactive peptides. In this study, collagen was prepared from antler ossified tissue via acetic acid and pepsin. Five different proteases were used to hydrolyze the collagen and the hydrolysate treated by neutrase (collagen peptide named ACP) showed the highest DPPH radical clearance rate. The extraction process of ACP was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 52 °C, a pH of 6.1, and an enzyme concentration of 3200 U/g, which resulted in the maximum DPPH clearance rate of 74.41 ± 0.48%. The peptides (ACP-3) with the strongest antioxidant activity were obtained after isolation and purification, and its DPPH free radical clearance rate was 90.58 ± 1.27%; at the same time, it exhibited good scavenging activity for ABTS, hydroxyl radical, and superoxide anion radical. The study investigated the protective effect of ACP-3 on oxidative damage in HaCaT cells. The findings revealed that all groups that received ACP-3 pretreatment exhibited increased activities of SOD, GSH-Px, and CAT compared to the model group. Furthermore, ACP-3 pretreatment reduced the levels of ROS and MDA in HaCaT cells subjected to H2O2-induced oxidative damage. These results suggest that collagen peptides derived from deer antler ossified tissue can effectively mitigate the oxidative damage caused by H2O2 in HaCaT cells, thereby providing a foundation for the utilization of collagen peptides in pharmaceuticals and cosmetics.
Keywords: antler ossified tissue; collagen peptide; antioxidant activity; oxidative damage antler ossified tissue; collagen peptide; antioxidant activity; oxidative damage
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MDPI and ACS Style

Chen, X.; Xia, P.; Zheng, S.; Li, Y.; Fang, J.; Ma, Z.; Zhang, L.; Zhang, X.; Hao, L.; Zhang, H. Antioxidant Peptides from the Collagen of Antler Ossified Tissue and Their Protective Effects against H2O2-Induced Oxidative Damage toward HaCaT Cells. Molecules 2023, 28, 6887. https://doi.org/10.3390/molecules28196887

AMA Style

Chen X, Xia P, Zheng S, Li Y, Fang J, Ma Z, Zhang L, Zhang X, Hao L, Zhang H. Antioxidant Peptides from the Collagen of Antler Ossified Tissue and Their Protective Effects against H2O2-Induced Oxidative Damage toward HaCaT Cells. Molecules. 2023; 28(19):6887. https://doi.org/10.3390/molecules28196887

Chicago/Turabian Style

Chen, Xi, Peijun Xia, Shuo Zheng, Yi Li, Jiayuan Fang, Ze Ma, Libo Zhang, Xunming Zhang, Linlin Hao, and Hong Zhang. 2023. "Antioxidant Peptides from the Collagen of Antler Ossified Tissue and Their Protective Effects against H2O2-Induced Oxidative Damage toward HaCaT Cells" Molecules 28, no. 19: 6887. https://doi.org/10.3390/molecules28196887

APA Style

Chen, X., Xia, P., Zheng, S., Li, Y., Fang, J., Ma, Z., Zhang, L., Zhang, X., Hao, L., & Zhang, H. (2023). Antioxidant Peptides from the Collagen of Antler Ossified Tissue and Their Protective Effects against H2O2-Induced Oxidative Damage toward HaCaT Cells. Molecules, 28(19), 6887. https://doi.org/10.3390/molecules28196887

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