Antioxidant Effects of Argan Oil and Olive Oil against Iron-Induced Oxidative Stress: In Vivo and In Vitro Approaches
Abstract
:1. Introduction
2. Results
2.1. Total Polyphenol Contents, Pigments Amounts, and Antioxidant Activities
2.2. IC50 of Iron
2.3. Argan Oil Protects T. pyriformis from Iron-Disturbed Antioxidant Activities
2.4. Argan Oil Protects the Liver from Iron-Disturbed Antioxidant Activities
2.5. Argan Oil Protects the Brain from Iron-Disturbed Antioxidant Activities
2.6. Argan Oil Protects the Kidneys from Iron-Disturbed Antioxidant Activities
Sample | SOD | GPx | MDA | GSH | |
---|---|---|---|---|---|
Tetrahymena pyriformis | C | 1.763 ± 0.346 | 3.572 ± 0.478 | 47.154 ± 4.173 | 8.273 ± 1.068 |
Iron | 2.992 ± 0.393 ** | 5.358 ± 0.604 * | 45.730 ± 7.603 | 10.521 ± 0.748 * | |
AO + Iron | 1.994 ± 0.173 + | 2.819 ± 0.239 ++ | 43.243 ± 5.873 | 7.319 ± 1.806 + | |
OO + Iron | 1.870 ± 0.322 + | 2.263 ± 0.450 ++ | 38.285 ± 6.185 | 4.584 ± 2.845 + | |
Liver | C | 9.728 ± 2.391 | 202.959 ± 73.156 | 6.737 ± 2.081 | 1.040 ± 0.151 |
Iron | 5.051 ± 0.550 * | 45.064 ± 8.087 * | 18.638 ± 1.113 *** | 0.817 ± 0.139 | |
AO + Iron | 8.104 ± 1.448 + | 110.850 ± 19.796 + | 7.442 ± 1.930 +++ | 0.772 ± 0.125 | |
OO + Iron | 8.400 ± 1.015 ++ | 163.332 ± 22.959 ++ | 9.469 ± 1.487 ++ | 0.536 ± 0.156 | |
Brain | C | 2.471 ± 0.604 | 114.339 ± 26.456 | 8.801 ± 0.564 | 2.905 ± 0.455 |
Iron | 3.197 ± 0.570 | 89.673 ± 20.172 | 5.227 ± 1.364 | 1.202 ± 0.212 ** | |
AO + Iron | 2.657 ± 0.284 | 92.365 ± 9.075 | 8.298 ± 5.173 | 1.938 ± 0.297 + | |
OO + Iron | 3.027 ± 0.665 | 23.030 ± 11.234 ++ | 9.696 ± 5.173 | 2.255 ± 0.211 ++ | |
Kidney | C | 2.747 ± 0.634 | 95.221 ± 29.860 | 5.920 ± 1.620 | 2.318 ± 0.268 |
Iron | 6.333 ± 0.933 ** | 32.101 ± 18.016 * | 5.242 ± 1.693 | 2.190 ± 0.112 | |
AO + Iron | 2.624 ± 0.401 ++ | 72.961 ± 8.967 + | 6.390 ± 1.010 | 1.134 ± 0.042 | |
OO + Iron | 3.216 ± 0.615 ++ | 94.682 ± 21.193 ++ | 4.820 ± 2.106 | 2.202 ± 0.185 +++ |
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Origin, Extraction, and Composition of Oils
Fatty Acids (%) | Argan Oil | Olive Oil |
---|---|---|
C14:0 (myristic acid) | 0.1 | 0.0 |
C15:0 (pentadecanoic acid) | <0.1 | ND a |
C16:0 (palmitic acid) | 12.6 | 9.7 |
C17:0 (margaric acid) | 0.1 | 0.0 |
C17:1 (heptadecenoic acid) | <0.1 | 0.1 |
C18:0 (stearic acid) | 5.8 | 2.6 |
C18:1 (oleic acid) | 46.3 | 73.6 |
C18:2 (linoleic acid) | 34.0 | 11.3 |
C18:3 (linolenic acid) | 0.1 | 1.0 |
C20:0 (arachidic acid) | 0.3 | 0.3 |
C20:1 (gadoleic acid) | 0.3 | 0.4 |
C22:0 (behenic acid) | 0.1 | 0.1 |
C24:0 (lignoceric acid) | ND a | 0.2 |
C18:1t | ND a | 0.02 |
C18:2t + C18:3t | ND a | 0.04 |
Argan Oil | Olive Oil | |
---|---|---|
Acidity (% as oleic acid) | 0.22 | 0.28 |
Peroxide value (meq O2/kg oil) | 2.1 | 3.2 |
K232 | ND a | 1.71 |
K270 | 0.13 | 0.15 |
ΔK | 0.003 | 0.00 |
Moisture and volatile matter (%) | 0.02 | 0.13 |
4.3. Total Polyphenol Contents
4.4. Determination of Chlorophyll and Carotenoid Contents
4.5. Antioxidant Activities
4.5.1. FRAP Assay
4.5.2. DPPH Assay
4.6. Culture and Treatments
4.6.1. Culture Medium
4.6.2. Iron Treatment
4.6.3. Argan Oil and Olive Oil Treatment
4.7. Animals and Experimental Design
4.8. Tetrahymena Pyriformis
4.9. Liver, Brain, and Kidney
4.10. Animals and Experimental Design
4.11. Oxidative Stress Markers and Antioxidants
4.11.1. Lipid Peroxidation
4.11.2. Glutathione
4.12. Enzymatic Activity Measurements
4.12.1. Glutathione Peroxidase
4.12.2. Superoxide Dismutase
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Groups | Treatment |
---|---|
Group I | Culture medium with the solvent ethanol |
Group II | Culture medium (as group I) supplemented with 0.1% of AO solubilized in ethanol |
Group III | Culture medium (as group I) supplemented with 0.1% of OO solubilized in ethanol |
Group IV | Culture medium (as group I) supplemented with iron at 1.849 mM dose dissolved in water |
Group V | Culture medium (as group I) and AO (as group II) |
Group VI | Culture medium (as group I) and AO (as group III) |
Groups | Treatment |
---|---|
Group I | A standard chow with the solvent acetone |
Group II | A standard chow (as group I) supplemented with 6% of AO solubilized in acetone |
Group III | A standard chow (as group I) supplemented with 6% of OO solubilized in acetone |
Group IV | The reference drug Tardyferon (iron sulphate 3.5 mg2+/liter) was dissolved in drinking water |
Group V | The animals received iron (as group IV) and AO (as group II) |
Group VI | The animals received iron (as group IV) and OO (as group III) |
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Bouchab, H.; Essadek, S.; El Kamouni, S.; Moustaid, K.; Essamadi, A.; Andreoletti, P.; Cherkaoui-Malki, M.; El Kebbaj, R.; Nasser, B. Antioxidant Effects of Argan Oil and Olive Oil against Iron-Induced Oxidative Stress: In Vivo and In Vitro Approaches. Molecules 2023, 28, 5924. https://doi.org/10.3390/molecules28155924
Bouchab H, Essadek S, El Kamouni S, Moustaid K, Essamadi A, Andreoletti P, Cherkaoui-Malki M, El Kebbaj R, Nasser B. Antioxidant Effects of Argan Oil and Olive Oil against Iron-Induced Oxidative Stress: In Vivo and In Vitro Approaches. Molecules. 2023; 28(15):5924. https://doi.org/10.3390/molecules28155924
Chicago/Turabian StyleBouchab, Habiba, Soukaina Essadek, Soufiane El Kamouni, Khadija Moustaid, Abdelkhalid Essamadi, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Riad El Kebbaj, and Boubker Nasser. 2023. "Antioxidant Effects of Argan Oil and Olive Oil against Iron-Induced Oxidative Stress: In Vivo and In Vitro Approaches" Molecules 28, no. 15: 5924. https://doi.org/10.3390/molecules28155924